Department of Orthopaedic Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
ACS Biomater Sci Eng. 2021 Dec 13;7(12):5739-5748. doi: 10.1021/acsbiomaterials.1c00747. Epub 2021 Nov 1.
Tendon adhesion formation is associated with the aberrant expression of many genes, and interfering with the expression of these genes can prevent adhesion and promote tendon repair. Recent studies have found that silencing the transforming growth factor β-1 () gene can reduce the occurrence of tendon adhesions. The development of tissue engineering and three-dimensional (3D) printing technology have provided new solutions for tendon repair. In this study, gene silencing microRNA (miRNA) based RNAi plasmid was loaded on a 3D tendon scaffold using 3D printing technology. experiments confirmed the sustained release of plasmid and the good biocompatibility of the printed tendon scaffold. Subsequently, the gene silencing plasmid loaded tendon scaffold was implanted in a chicken tendon defect model to evaluate the effect of the scaffold . The results from biomechanical tests and histological examinations showed that the scaffold not only promoted tendon regeneration but also prevented tendon adhesion, which was conducive to the recovery of biofunction. Evaluation of protein expression showed that the loaded plasmids prevented tendon adhesion and promoted tendon functional repair via silencing of the gene.
肌腱粘连的形成与许多基因的异常表达有关，干扰这些基因的表达可以防止粘连并促进肌腱修复。最近的研究发现，沉默转化生长因子-β1（）基因可以减少肌腱粘连的发生。组织工程和三维（3D）打印技术的发展为肌腱修复提供了新的解决方案。在这项研究中，使用 3D 打印技术将基于基因沉默微小 RNA（miRNA）的 RNAi 质粒加载到 3D 肌腱支架上。实验证实了质粒的持续释放和打印肌腱支架的良好生物相容性。随后，将负载基因沉默质粒的肌腱支架植入鸡肌腱缺损模型中，以评估支架的效果。生物力学测试和组织学检查结果表明，该支架不仅促进了肌腱再生，而且防止了肌腱粘连，有利于生物功能的恢复。蛋白表达的评估表明，负载的质粒通过沉默基因预防肌腱粘连并促进肌腱功能修复。
