Carvalho Carolina S, de Oliveira Marina Elisa, Rodriguez-Castro Karen Giselle, Saranholi Bruno H, Galetti Pedro M
Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil.
Instituto Tecnológico Vale, Belém, PA, Brazil.
Mol Ecol Resour. 2022 May;22(4):1262-1273. doi: 10.1111/1755-0998.13543. Epub 2021 Nov 11.
Environmental DNA (eDNA) and invertebrate-derived DNA (iDNA) have been increasingly recognized as powerful tools for biodiversity assessment and conservation management. However, eDNA/iDNA efficiency for vertebrate diversity assessment remains uncertain, and comparisons to conventional methods are still rare. Through a meta-analysis of previously published vertebrate diversity surveys, we compared the efficiency of eDNA/iDNA against conventional methods across several types of samplers, vertebrate groups, and locations (tropical vs. temperate zones). We also assess eDNA/iDNA efficiency to estimate relative abundance or biomass over different molecular methods (qPCR and metabarcoding) and type of experiment (in the laboratory or in the field). We showed that for water sampler, fish as a target species, and studies achieved in temperate zones, eDNA presents lower risk of not detecting a species or a site with a target species than conventional methods. These results show that eDNA is an efficient tool to assess fish diversity. Moreover, eDNA data presents positive correlation with fish abundance or biomass. However, such correlation was higher in laboratory experiments than in the field. For the other samplers, vertebrate groups, and in tropical zones we were not able to draw general conclusion, highlighting the urgency of conducting more comparative studies.
环境DNA（eDNA）和无脊椎动物衍生DNA（iDNA）已日益被视为生物多样性评估和保护管理的有力工具。然而，eDNA/iDNA用于脊椎动物多样性评估的效率仍不确定，与传统方法的比较也仍然很少见。通过对先前发表的脊椎动物多样性调查进行荟萃分析，我们比较了eDNA/iDNA与传统方法在几种采样器类型、脊椎动物群体和地点（热带与温带）方面的效率。我们还评估了eDNA/iDNA在不同分子方法（定量聚合酶链反应和宏条形码技术）和实验类型（实验室或野外）下估计相对丰度或生物量的效率。我们发现，对于水样采集器、以鱼类作为目标物种以及在温带地区开展的研究，与传统方法相比，eDNA未检测到某一物种或某一有目标物种的地点的风险更低。这些结果表明，eDNA是评估鱼类多样性的有效工具。此外，eDNA数据与鱼类丰度或生物量呈正相关。然而，这种相关性在实验室实验中比在野外更高。对于其他采样器、脊椎动物群体以及在热带地区，我们无法得出一般性结论，这凸显了开展更多比较研究的紧迫性。
