College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
BMC Genom Data. 2021 Nov 1;22(1):46. doi: 10.1186/s12863-021-01003-z.
With high-efficient water-use and drought tolerance, broomcorn millet has emerged as a candidate for food security. To promote its research process for molecular breeding and functional research, a comprehensive genome resource is of great importance.
Herein, we constructed a BAC library for broomcorn millet, generated BAC end sequences based on the clone-array pooled shotgun sequencing strategy and Illumina sequencing technology, and integrated BAC clones into genome by a novel pipeline for BAC end profiling. The BAC library consisted of 76,023 clones with an average insert length of 123.48 Kb, covering about 9.9-fold of the 850 Mb genome. Of 9216 clones tested using our pipeline, 8262 clones were mapped on the broomcorn millet cultivar longmi4 genome. These mapped clones covered 308 of the 829 gaps left by the genome. To our knowledge, this is the only BAC resource for broomcorn millet.
We constructed a high-quality BAC libraray for broomcorn millet and designed a novel pipeline for BAC end profiling. BAC clones can be browsed and obtained from our website ( http://eightstarsbio.com/gresource/JBrowse-1.16.5/index.html ). The high-quality BAC clones mapped on genome in this study will provide a powerful genomic resource for genome gap filling, complex segment sequencing, FISH, functional research and genetic engineering of broomcorn millet.
谷子具有高效用水和耐旱的特性，已成为保障粮食安全的候选作物。为了促进谷子的分子育种和功能研究，全面的基因组资源至关重要。
本研究构建了谷子的 BAC 文库，采用克隆数组池化测序策略和 Illumina 测序技术生成 BAC 末端序列，并通过新型 BAC 末端图谱构建流程将 BAC 克隆整合到基因组中。该 BAC 文库包含 76023 个克隆，平均插入片段长度为 123.48 Kb，覆盖约 850 Mb 基因组的 9.9 倍。使用我们的流程对 9216 个克隆进行了测试，其中 8262 个克隆被映射到谷子品种龙米 4 号基因组上。这些映射的克隆覆盖了基因组中 308 个缺口。据我们所知，这是谷子唯一的 BAC 资源。
我们构建了高质量的谷子 BAC 文库，并设计了新型的 BAC 末端图谱构建流程。BAC 克隆可以从我们的网站（http://eightstarsbio.com/gresource/JBrowse-1.16.5/index.html）浏览和获取。本研究中映射到基因组上的高质量 BAC 克隆将为谷子基因组缺口填充、复杂片段测序、FISH、功能研究和遗传工程提供强大的基因组资源。
