Green Michael R, Sambrook Joseph
Cold Spring Harb Protoc. 2021 Nov 1;2021(11):2021/11/pdb.top101170. doi: 10.1101/pdb.top101170.
Plasmids occupy a place of honor in molecular cloning: They were used in the first recombinant DNA experiments and, 40 or more years later, they remain as the carriage horses of molecular cloning. After almost half a century of sequential improvement in design, today's plasmid vectors are available in huge variety, are often optimized for specific purposes, and bear only passing resemblance to their forebears. Here, various features of plasmid vectors and methods for transforming cells are introduced.
它们被用于首次重组DNA实验，并且在40多年后的今天，它们仍然是分子克隆的运载工具。经过近半个世纪的设计不断改进，如今的质粒载体种类繁多，常常针对特定目的进行了优化，与它们的前身仅有些许相似之处。在此，将介绍质粒载体的各种特性以及细胞转化方法。
