Sunitha I, Slobin L I
Biochem Biophys Res Commun. 1987 Apr 29;144(2):560-8. doi: 10.1016/s0006-291x(87)80003-7.
A system consisting of 40-80S messenger ribonucleoprotein particles (mRNP) from stationary Friend erythroleukemia (FEL) cells was used to investigate the stability of mRNA in vitro. The majority of mRNP mRNAs were found to be stable when incubated for periods of up to ninety minutes at 37 degrees. Nonetheless, many mRNAs are greatly reduced in abundance, including ones for eucaryotic elongation factor Tu (eEF-Tu) and the 73-78 kDa polypeptide commonly found in association with the poly(A) tails of mRNA. A divalent cation dependent ribonuclease (probably an endoribonuclease) could be washed off mRNP by treatment of the particles with 0.5M NaCl. The mRNAs contained in the resultant salt washed mRNPs, including eEF-Tu, were stable when incubated in vitro.
使用来自静止期弗氏红白血病（FEL）细胞的由40 - 80S信使核糖核蛋白颗粒（mRNP）组成的系统来体外研究mRNA的稳定性。当在37℃孵育长达90分钟时，发现大多数mRNP mRNA是稳定的。然而，许多mRNA的丰度大幅降低，包括真核延伸因子Tu（eEF - Tu）以及通常与mRNA的聚（A）尾相关联的73 - 78 kDa多肽的mRNA。通过用0.5M NaCl处理颗粒，可以将一种二价阳离子依赖性核糖核酸酶（可能是一种内切核糖核酸酶）从mRNP上洗脱下来。所得经盐洗涤的mRNP中所含的mRNA，包括eEF - Tu，在体外孵育时是稳定的。
