• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

小鼠真核延伸因子Tu基因在小鼠红白血病细胞分化过程中的表达

Expression of a gene for mouse eucaryotic elongation factor Tu during murine erythroleukemic cell differentiation.

作者信息

Roth W W, Bragg P W, Corrias M V, Reddy N S, Dholakia J N, Wahba A J

机构信息

Department of Biochemistry, University of Mississippi Medical Center, Jackson 39216.

出版信息

Mol Cell Biol. 1987 Nov;7(11):3929-36. doi: 10.1128/mcb.7.11.3929-3936.1987.

DOI:10.1128/mcb.7.11.3929-3936.1987
PMID:3481036
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368061/
Abstract

The eucaryotic elongation factor Tu (eEF-Tu) is a single polypeptide with an approximate Mr of 53,000. During protein synthesis eEF-Tu promotes the binding of aminoacyl-tRNA to the ribosome. To study the expression of the gene(s) for this factor, a genomic clone was isolated that contains a mouse eEF-Tu gene. We screened a phage genomic library with a synthetic oligonucleotide probe complementary to a region of the Saccharomyces cerevisiae and Artemia sp. eEF-Tu genes which codes for an area that is highly conserved between both yeast and Artemia sp. eEF-Tu. From approximately 75,000 phage plaques we obtained five isolates with apparently identical inserts. All five clones contained a 3.8-kilobase EcoRI fragment that hybridized to additional oligonucleotide probes corresponding to different conserved regions of eEF-Tu. We sequenced the 5' end of one genomic clone and determined the length of the cloned fragment that was protected by eEF-Tu mRNA in S1 nuclease protection assays. A quantitative S1 nuclease protection assay was used to compare the relative steady-state levels of eEF-Tu mRNA in total mRNA in total RNA isolated from hexamethylene-bisacetamide-induced murine erythroleukemia cells. The results show a dramatic reduction in the steady-state level of eEF-Tu mRNA as differentiation proceeds. A similar reduction in transcription of eEF-Tu mRNA was observed in isolated nuclei. Finally, we examined the in vivo synthesis of eEF-Tu during differentiation and found that it declined in a manner parallel to the decline in the steady-state level of eEF-Tu mRNA. In addition, we have isolated and sequenced a cDNA clone for mouse eEF-Tu. The derived amino acid sequence is compared with sequences from other eucaryotes.

摘要

真核生物延伸因子Tu（eEF-Tu）是一种单条多肽链，分子量约为53,000。在蛋白质合成过程中，eEF-Tu促进氨酰tRNA与核糖体的结合。为了研究该因子基因的表达，分离出了一个包含小鼠eEF-Tu基因的基因组克隆。我们用与酿酒酵母和卤虫属eEF-Tu基因某区域互补的合成寡核苷酸探针筛选噬菌体基因组文库，该区域编码酵母和卤虫属eEF-Tu之间高度保守的区域。从大约75,000个噬菌斑中，我们获得了五个插入片段明显相同的分离株。所有五个克隆都含有一个3.8千碱基的EcoRI片段，该片段与对应于eEF-Tu不同保守区域的其他寡核苷酸探针杂交。我们对一个基因组克隆的5'端进行了测序，并在S1核酸酶保护试验中确定了受eEF-Tu mRNA保护的克隆片段的长度。使用定量S1核酸酶保护试验比较了从六亚甲基双乙酰胺诱导的小鼠红白血病细胞中分离的总RNA中eEF-Tu mRNA的相对稳态水平。结果表明，随着分化的进行，eEF-Tu mRNA的稳态水平急剧下降。在分离的细胞核中也观察到eEF-Tu mRNA转录的类似下降。最后，我们检查了分化过程中eEF-Tu的体内合成情况，发现其下降方式与eEF-Tu mRNA稳态水平的下降平行。此外，我们还分离并测序了小鼠eEF-Tu的cDNA克隆。将推导的氨基酸序列与其他真核生物的序列进行了比较。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a35/368061/9283c977b516/molcellb00083-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a35/368061/d92da2d41bac/molcellb00083-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a35/368061/86ee7e511cbe/molcellb00083-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a35/368061/ee8830315c1b/molcellb00083-0079-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a35/368061/9283c977b516/molcellb00083-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a35/368061/d92da2d41bac/molcellb00083-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a35/368061/86ee7e511cbe/molcellb00083-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a35/368061/ee8830315c1b/molcellb00083-0079-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a35/368061/9283c977b516/molcellb00083-0080-a.jpg

相似文献

1
Expression of a gene for mouse eucaryotic elongation factor Tu during murine erythroleukemic cell differentiation.小鼠真核延伸因子Tu基因在小鼠红白血病细胞分化过程中的表达
Mol Cell Biol. 1987 Nov;7(11):3929-36. doi: 10.1128/mcb.7.11.3929-3936.1987.
2
Translational repression of mRNA for eucaryotic elongation factors in Friend erythroleukemia cells.Friend红白血病细胞中真核生物延伸因子mRNA的翻译抑制
Eur J Biochem. 1984 Nov 15;145(1):143-50. doi: 10.1111/j.1432-1033.1984.tb08533.x.
3
The expression of a gene for eukaryotic elongation factor Tu in Artemia during development. Translation of poly(A)+ RNA and the use of a synthetic oligonucleotide to detect the presence of eukaryotic elongation factor Tu-specific mRNA.卤虫发育过程中真核延伸因子 Tu 基因的表达。聚腺苷酸（poly(A)+）RNA 的翻译以及使用合成寡核苷酸检测真核延伸因子 Tu 特异性 mRNA 的存在。
J Biol Chem. 1985 Dec 25;260(30):16347-53.
4
Regulation of the utilization of mRNA for eucaryotic elongation factor Tu in Friend erythroleukemia cells.Friend红白血病细胞中真核延伸因子Tu的mRNA利用调控
Mol Cell Biol. 1987 Feb;7(2):687-97. doi: 10.1128/mcb.7.2.687-697.1987.
5
The stability of mRNA for eucaryotic elongation factor Tu in Friend erythroleukemia cells varies with growth rate.弗氏红白血病细胞中真核延伸因子Tu的mRNA稳定性随生长速率而变化。
Mol Cell Biol. 1988 Mar;8(3):1085-92. doi: 10.1128/mcb.8.3.1085-1092.1988.
6
Functional and structural studies on a tryptic fragment of eucaryotic elongation factor Tu from rabbit reticulocytes.兔网织红细胞真核延伸因子Tu胰蛋白酶片段的功能与结构研究
Biochemistry. 1981 Sep 29;20(20):5761-7. doi: 10.1021/bi00523a019.
7
The role of eucaryotic factor Tu in protein synthesis. The measurement of the elongation factor Tu content of rabbit reticulocytes and other mammalian cells by a sensitive radioimmunoassay.真核因子Tu在蛋白质合成中的作用。通过灵敏的放射免疫测定法测定兔网织红细胞和其他哺乳动物细胞中延伸因子Tu的含量。
Eur J Biochem. 1980 Sep;110(2):555-63. doi: 10.1111/j.1432-1033.1980.tb04898.x.
8
Binding of eucaryotic elongation factor Tu to nucleic acids.真核生物延伸因子Tu与核酸的结合。
J Biol Chem. 1983 Apr 25;258(8):4895-900.
9
An in vitro system derived from Friend erythroleukemia cells to study messenger RNA stability.一种源自弗瑞德红白血病细胞的体外系统，用于研究信使核糖核酸的稳定性。
Biochem Biophys Res Commun. 1987 Apr 29;144(2):560-8. doi: 10.1016/s0006-291x(87)80003-7.
10
Isolation and mapping of a gene for protein synthesis initiation factor 4A and its expression during differentiation of murine erythroleukemia cells.蛋白质合成起始因子4A基因的分离、定位及其在小鼠红白血病细胞分化过程中的表达
Gene. 1988 Oct 30;70(2):231-43. doi: 10.1016/0378-1119(88)90195-3.

引用本文的文献

1
Repression of global protein synthesis by Eif1a-like genes that are expressed specifically in the two-cell embryos and the transient Zscan4-positive state of embryonic stem cells.在两细胞胚胎中特异性表达的 Eif1a 样基因和胚胎干细胞的短暂 Zscan4 阳性状态抑制了全球蛋白质合成。
DNA Res. 2013 Aug;20(4):391-402. doi: 10.1093/dnares/dst018. Epub 2013 May 5.
2
Phosphoglycerylethanolamine posttranslational modification of plant eukaryotic elongation factor 1alpha.植物真核延伸因子1α的磷酸甘油乙醇胺翻译后修饰
Plant Physiol. 1998 Jul;117(3):949-60. doi: 10.1104/pp.117.3.949.
3
Isolation, characterization and mRNA expression of four cDNAs encoding translation elongation factor 1A from rice (Oryza sativa L.).

本文引用的文献

1
Induction of hemoglobin synthesis by xylosyladenine in murine erythroleukemia cells. Metabolism of xylosyladenine and effects on transmethylation.
J Biol Chem. 1981 Dec 25;256(24):12705-9.
2
The role of eucaryotic factor Tu in protein synthesis. The measurement of the elongation factor Tu content of rabbit reticulocytes and other mammalian cells by a sensitive radioimmunoassay.真核因子Tu在蛋白质合成中的作用。通过灵敏的放射免疫测定法测定兔网织红细胞和其他哺乳动物细胞中延伸因子Tu的含量。
Eur J Biochem. 1980 Sep;110(2):555-63. doi: 10.1111/j.1432-1033.1980.tb04898.x.
3
Protein synthesis in brine shrimp embryos. Regulation of the formation of the ternary complex (Met-tRNAf X eIF-2 X GTP) by two purified protein factors and phosphorylation of Artemia eIF-2.卤虫胚胎中的蛋白质合成。两种纯化的蛋白质因子对三元复合物（Met-tRNAf·eIF-2·GTP）形成的调节以及卤虫eIF-2的磷酸化作用。
水稻（Oryza sativa L.）中四个编码翻译延伸因子1A的cDNA的分离、特性鉴定及mRNA表达分析
Plant Mol Biol. 1998 Jan;36(1):137-48. doi: 10.1023/a:1005960721762.
4
Cloning and characterization of a TEF gene for elongation factor 1 alpha from the yeast Arxula adeninivorans.来自嗜腺阿舒囊酵母的延伸因子1α的TEF基因的克隆与特性分析
Curr Genet. 1995 Sep;28(4):360-6. doi: 10.1007/BF00326434.
5
Rapamycin selectively represses translation of the "polypyrimidine tract" mRNA family.雷帕霉素选择性抑制“多嘧啶序列”mRNA家族的翻译。
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4441-5. doi: 10.1073/pnas.91.10.4441.
6
Protein synthesis elongation factor EF-1 alpha expression and longevity in Drosophila melanogaster.
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4199-203. doi: 10.1073/pnas.91.10.4199.
7
Detection of protein similarities using nucleotide sequence databases.利用核苷酸序列数据库检测蛋白质相似性。
Nucleic Acids Res. 1988 Jul 11;16(13):6191-204. doi: 10.1093/nar/16.13.6191.
8
Two genes encode related cytoplasmic elongation factors 1 alpha (EF-1 alpha) in Drosophila melanogaster with continuous and stage specific expression.在黑腹果蝇中，有两个基因编码相关的细胞质延伸因子1α（EF-1α），它们具有持续表达和阶段特异性表达。
Nucleic Acids Res. 1988 Apr 25;16(8):3175-94. doi: 10.1093/nar/16.8.3175.
9
Retropseudogenes constitute the major part of the human elongation factor 1 alpha gene family.反转录假基因构成了人类延伸因子1α基因家族的主要部分。
Nucleic Acids Res. 1990 Mar 25;18(6):1513-6. doi: 10.1093/nar/18.6.1513.
10
Characterization of a plasmid involved with cointegrate formation and lactose metabolism in Lactococcus lactis subsp. lactis OZS1.乳酸乳球菌乳亚种OZS1中与共整合体形成及乳糖代谢相关的质粒的特性分析
Arch Microbiol. 1990;154(6):560-5. doi: 10.1007/BF00248837.
Eur J Biochem. 1981 Jul;117(3):543-51.
4
Genes for elongation factor EF-1 alpha in the brine shrimp Artemia.卤虫中延伸因子EF-1α的基因。
FEBS Lett. 1983 Jul 4;157(2):295-9. doi: 10.1016/0014-5793(83)80564-x.
5
Histone mRNA concentrations are regulated at the level of transcription and mRNA degradation.组蛋白mRNA的浓度在转录和mRNA降解水平上受到调控。
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1849-53. doi: 10.1073/pnas.80.7.1849.
6
Molecular aspects of development in the brine shrimp Artemia.卤虫发育的分子机制
Prog Nucleic Acid Res Mol Biol. 1984;31:221-65. doi: 10.1016/s0079-6603(08)60379-7.
7
Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae.酿酒酵母翻译延伸因子EF-1α编码的两个基因的鉴定。
EMBO J. 1984 Dec 20;3(13):3311-5. doi: 10.1002/j.1460-2075.1984.tb02295.x.
8
Polypeptide chain elongation factor 1 alpha (EF-1 alpha) from yeast: nucleotide sequence of one of the two genes for EF-1 alpha from Saccharomyces cerevisiae.酵母的多肽链延伸因子1α（EF-1α）：酿酒酵母EF-1α两个基因之一的核苷酸序列。
EMBO J. 1984 Aug;3(8):1825-30. doi: 10.1002/j.1460-2075.1984.tb02053.x.
9
Cross-linking of tRNA at two different sites of the elongation factor Tu.在延伸因子Tu的两个不同位点处tRNA的交联。
Proc Natl Acad Sci U S A. 1984 Jul;81(13):3969-72. doi: 10.1073/pnas.81.13.3969.
10
The primary structure of elongation factor EF-1 alpha from the brine shrimp Artemia.卤虫伸长因子EF-1α的一级结构。
EMBO J. 1984 May;3(5):1109-13. doi: 10.1002/j.1460-2075.1984.tb01937.x.