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Friend红白血病细胞中真核延伸因子Tu的mRNA利用调控

Regulation of the utilization of mRNA for eucaryotic elongation factor Tu in Friend erythroleukemia cells.

作者信息

Rao T R, Slobin L I

出版信息

Mol Cell Biol. 1987 Feb;7(2):687-97. doi: 10.1128/mcb.7.2.687-697.1987.

DOI:10.1128/mcb.7.2.687-697.1987
PMID:2434834
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365125/
Abstract

When Friend erythroleukemia cells were allowed to grow to stationary phase (2 X 10(6) to 3 X 10(6) cells per ml), approximately 60% of the mRNA for eucaryotic elongation factor Tu (eEF-Tu) sedimented at less than or equal to 80S, and most of the remaining factor mRNA was associated with small polysomes. Under the same growth conditions, greater than 90% of the mRNA for eucaryotic initiation factor 4A remained associated with polysomes. The association of eEF-Tu mRNA with polysomes changed dramatically when stationary-phase cells were treated with fresh medium. After 1 h in fresh medium, approximately 90% of eEF-Tu mRNA in Friend cells was found in heavy polysomes. Associated with the shift of eEF-Tu mRNA into heavy polysomes, we found at least a 2.6-fold increase in the synthesis of eEF-Tu in vivo as well as a remarkable 40% decrease in the total amount of eEF-Tu mRNA per cell. Our data raise the possibility that eEF-Tu mRNA that has accumulated in ribonucleoprotein particles in stationary-phase cells is degraded rather than reutilized for eEF-Tu synthesis.

摘要

当Friend红白血病细胞生长至稳定期（每毫升2×10⁶至3×10⁶个细胞）时，真核延伸因子Tu（eEF-Tu）的mRNA约60%沉降系数小于或等于80S，其余大部分因子mRNA与小多核糖体相关。在相同生长条件下，真核起始因子4A的mRNA超过90%仍与多核糖体相关。当用新鲜培养基处理稳定期细胞时，eEF-Tu mRNA与多核糖体的结合发生了显著变化。在新鲜培养基中培养1小时后，Friend细胞中约90%的eEF-Tu mRNA存在于重多核糖体中。随着eEF-Tu mRNA向重多核糖体的转移，我们发现体内eEF-Tu的合成至少增加了2.6倍，且每个细胞中eEF-Tu mRNA的总量显著减少了40%。我们的数据提出了一种可能性，即稳定期细胞核糖核蛋白颗粒中积累的eEF-Tu mRNA被降解，而非重新用于eEF-Tu的合成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/08154e6155ab/molcellb00074-0139-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/75cc880c523c/molcellb00074-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/2fd64a8acb9a/molcellb00074-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/c715ab637a11/molcellb00074-0135-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/1f7962058bdc/molcellb00074-0135-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/9aac1dc7e101/molcellb00074-0136-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/b7ada31829d7/molcellb00074-0136-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/2d066a6bdb34/molcellb00074-0137-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/c95feab9601c/molcellb00074-0137-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/5d827dcc7543/molcellb00074-0137-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/22c5b6a2b5a7/molcellb00074-0138-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/08154e6155ab/molcellb00074-0139-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/75cc880c523c/molcellb00074-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/2fd64a8acb9a/molcellb00074-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/c715ab637a11/molcellb00074-0135-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/1f7962058bdc/molcellb00074-0135-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/9aac1dc7e101/molcellb00074-0136-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/b7ada31829d7/molcellb00074-0136-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/2d066a6bdb34/molcellb00074-0137-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/c95feab9601c/molcellb00074-0137-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/5d827dcc7543/molcellb00074-0137-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/22c5b6a2b5a7/molcellb00074-0138-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5578/365125/08154e6155ab/molcellb00074-0139-a.jpg

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Polysomal and non-polysomal messenger RNA in neuroblastoma cells. Lack of correlation between polyadenylation or initiation efficiency and messenger RNA location.神经母细胞瘤细胞中的多聚核糖体信使核糖核酸和非多聚核糖体信使核糖核酸。多聚腺苷酸化或起始效率与信使核糖核酸位置之间缺乏相关性。
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具有5'-末端嘧啶序列的脊椎动物mRNA是静止细胞中翻译抑制的候选物：翻译顺式调控元件的表征。
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The stability of mRNA for eucaryotic elongation factor Tu in Friend erythroleukemia cells varies with growth rate.弗氏红白血病细胞中真核延伸因子Tu的mRNA稳定性随生长速率而变化。
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Translational initiation factor expression and ribosomal protein gene expression are repressed coordinately but by different mechanisms in murine lymphosarcoma cells treated with glucocorticoids.在糖皮质激素处理的小鼠淋巴肉瘤细胞中，翻译起始因子表达和核糖体蛋白基因表达受到协同抑制，但机制不同。
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