Aimone-Gastin I, Cable S, Keller J M, Bigard M A, Champigneulle B, Gaucher P, Gueant J L, Dauça M
Laboratoire de Biologie Cellulaire du Développement, Université de Nancy I, Faculté des Sciences, France.
Dig Dis Sci. 1994 Oct;39(10):2177-85. doi: 10.1007/BF02090368.
The etiology and pathogenesis of Crohn's disease, a chronic inflammatory bowel pathology, have not been elucidated yet. In particular, the behavior of peroxisomes in inflamed colonic mucosa has not been investigated despite their important role in cellular oxidative metabolism. Using cytochemistry at the ultrastructural level, we have observed these catalase-positive organelles. In addition, biochemical analyses have revealed the specific activities of catalase and cyanide-insensitive acyl-CoA oxidase. Mucosal biopsy specimens from inflamed and noninflamed areas of Crohn's patients were compared to control biopsies. We found that Crohn's disease was marked by an important diminution in the peroxisomal frequency per cell unit area. If catalase activity was not affected by this pathology, cyanide-insensitive acyl-CoA oxidase, an enzyme of the peroxisomal beta-oxidation system, was found diminished in inflamed and in noninflamed areas. In conclusion, our results showed that Crohn's disease is accompanied by peroxisomal modifications but the number and the enzyme activities of colonic peroxisomes are less deeply altered in Crohn's disease than during neoplasia. This fact suggests that a relation may exist between the degree of peroxisomal deficiency and the clinical severity of colonic disease.
克罗恩病是一种慢性炎症性肠病，其病因和发病机制尚未阐明。特别是，尽管过氧化物酶体在细胞氧化代谢中发挥重要作用，但在炎症性结肠黏膜中的行为尚未得到研究。利用超微结构水平的细胞化学方法，我们观察到了这些过氧化氢酶阳性细胞器。此外，生化分析揭示了过氧化氢酶和对氰化物不敏感的酰基辅酶A氧化酶的比活性。将克罗恩病患者炎症和非炎症区域的黏膜活检标本与对照活检标本进行比较。我们发现，克罗恩病的特征是每单位细胞面积的过氧化物酶体频率显著降低。如果过氧化氢酶活性不受这种病理状态的影响，那么过氧化物酶体β氧化系统的一种酶——对氰化物不敏感的酰基辅酶A氧化酶，在炎症和非炎症区域均减少。总之，我们的结果表明，克罗恩病伴有过氧化物酶体改变，但与肿瘤形成相比，克罗恩病中结肠过氧化物酶体的数量和酶活性改变程度较小。这一事实表明，过氧化物酶体缺乏程度与结肠疾病的临床严重程度之间可能存在关联。
