KHARKIV NATIONAL MEDICAL UNIVERSITY, KHARKIV, UKRAINE.
Wiad Lek. 2021;74(9 cz 1):2109-2111.
The aim: Study of antibacterial activity of the preparations, containing antiseptic dioxidine and antibiotic levofloxacin in vitro on standard strains of main optional-anaerobic pathogens of purulent-inflammatory processes of surgical wounds S. aureus, E. coli, P. aeruginosa and definition of more effective ones on them.
Materials and methods: Solutions of dioxidine 1.2 %, dioxidine 1.2% with decamethaxin, Dioxisole, water soluble ointment with dioxidine 1.2% and levofloxacin 0.1% with decamethaxin were used in experiment. Antibacterial activity was studied on standard strains of S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Distinguishing and identification of pure cultures of bacteria was done according to generally accepted microbiological methods. Determination of purulent-inflammatory processes pathogens sensitivity was done by disco-diffuse method on Mueller-Hinton medium. Antibacterial activity of solutions and ointments was studied with the help of agar diffusion method ("well" method) according to methodic recommendations. Each investigation was repeated 6 times. Method of variation statistics was used for the research results analysis.
Results: All antibacterial preparations under study are effective and highly effective on S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Solution with 1.2 % dioxidine with decamethaxin and ointment with 0.1 % levofloxacin and decamethaxin have larger growth retardation zones towards S. aureus and P. aeruginosa. E. coli strains are more sensitive to the solution of Dioxisole and ointment with 1.2 % dioxidine.
Conclusions: All strains are sensitive, most of them are highly sensitive, up to 5 antibacterial preparations under study in vitro.
研究含防腐剂二氧啶和抗生素左氧氟沙星的制剂对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌等化脓性炎症手术伤口主要选择性厌氧菌标准菌株的体外抗菌活性，并确定对它们更有效的制剂。
实验中使用了 1.2%二氧啶溶液、1.2%二氧啶与癸甲溴铵溶液、二氧异恶唑、1.2%二氧啶水溶性软膏和 0.1%左氧氟沙星与癸甲溴铵软膏。在标准菌株金黄色葡萄球菌 ATCC 25923、大肠杆菌 ATCC 25922、铜绿假单胞菌 ATCC 27853 上研究了抗菌活性。根据公认的微生物学方法对细菌纯培养物进行了鉴别和鉴定。在 Mueller-Hinton 培养基上采用弥散盘法测定脓性炎症病原菌的敏感性。利用琼脂扩散法（“孔”法）研究了溶液和软膏的抗菌活性，根据方法建议进行。每项研究重复 6 次。使用变异统计方法对研究结果进行分析。
所有研究中的抗菌制剂对金黄色葡萄球菌 ATCC 25923、大肠杆菌 ATCC 25922、铜绿假单胞菌 ATCC 27853 均有效且高度有效。1.2%二氧啶与癸甲溴铵溶液和 0.1%左氧氟沙星与癸甲溴铵软膏对金黄色葡萄球菌和铜绿假单胞菌的生长抑制区更大。大肠杆菌菌株对二氧异恶唑溶液和 1.2%二氧啶软膏更敏感。
所有菌株均敏感，其中大部分高度敏感，研究中的 5 种抗菌制剂在体外均有效。
