Hubert Jan, Nesvorna Marta, Klimov Pavel B, Erban Tomas, Sopko Bruno, Dowd Scot E, Scully Erin D
Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czechia.
Crop Research Institute, Prague, Czechia.
mSystems. 2021 Dec 21;6(6):e0091621. doi: 10.1128/mSystems.00916-21. Epub 2021 Nov 2.
is inhabited by an intracellular bacterium, Using correlations between host and symbiont gene expression profiles, we identified several important molecular pathways that potentially regulate/facilitate their interactions. The expression of genes collectively explained 95% of the variation in the expression of mite genes assigned to pathways for phagocytosis, apoptosis, the MAPK signaling cascade, endocytosis, the tumor necrosis factor (TNF) pathway, the transforming growth factor beta (TGF-β) pathway, lysozyme, and the Toll/Imd pathway. In addition, expression of mite genes explained 76% of the variability in gene expression. In particular, the expression of the genes encoding the signaling molecules BamD, LepA, SymE, and VirD4 was either positively or negatively correlated with the expression levels of mite genes involved in endocytosis, phagocytosis, and apoptosis. We also found that possesses a complete biosynthetic pathway for lipoic acid and may provide lipoate, but not biotin, to mites. gene expression collectively explained 84% of the variation in expression related to several core mite metabolic pathways, and, most notably, a negative correlation was observed between bacterial gene expression and expression of mite genes assigned to the glycolysis and citric acid cycle pathways. Furthermore, we showed that gene expression is correlated with expression levels of genes associated with terpenoid backbone biosynthesis. This pathway is important for the synthesis of pheromones, thus providing an opportunity for to influence mite reproductive behavior to facilitate transmission of the bacterium. Overall, our study provided correlational gene expression data that can be useful for future research on mite- interactions. The molecular mechanisms of mite-symbiont interactions and their impacts on human health are largely unknown. Astigmatid mites, such as house dust and stored-product mites, are among the most significant allergen sources worldwide. Although mites themselves are the main allergen sources, recent studies have indicated that mite-associated microbiomes may have implications for allergen production and human health. The major medically important house dust mite, D. farinae, is known to harbor a highly abundant intracellular bacterium belonging to the genus . Expression analysis of the mite and symbiont genes can identify key mite molecular pathways that facilitate interactions with this endosymbiont and possibly shed light on how this bacterium affects mite allergen production and physiology in general.
它被一种细胞内细菌所占据。通过宿主和共生体基因表达谱之间的相关性，我们确定了几个潜在调节/促进它们相互作用的重要分子途径。这些基因的表达共同解释了分配到吞噬作用、细胞凋亡、丝裂原活化蛋白激酶（MAPK）信号级联、内吞作用、肿瘤坏死因子（TNF）途径、转化生长因子β（TGF-β）途径、溶菌酶和Toll/Imd途径的螨类基因表达变异的95%。此外，螨类基因的表达解释了基因表达变异的76%。特别是，编码信号分子BamD、LepA、SymE和VirD4的基因表达与参与内吞作用、吞噬作用和细胞凋亡的螨类基因表达水平呈正相关或负相关。我们还发现它拥有完整的硫辛酸生物合成途径，可能为螨类提供硫辛酸，但不提供生物素。基因表达共同解释了与几种核心螨类代谢途径相关的表达变异的84%，最值得注意的是，观察到细菌基因表达与分配到糖酵解和柠檬酸循环途径的螨类基因表达之间存在负相关。此外，我们表明基因表达与类萜骨架生物合成相关基因的表达水平相关。该途径对信息素的合成很重要，从而为影响螨类生殖行为以促进细菌传播提供了机会。总体而言，我们的研究提供了相关的基因表达数据，可用于未来关于螨类与相互作用的研究。螨类与共生体相互作用的分子机制及其对人类健康的影响在很大程度上尚不清楚。粉螨，如屋尘螨和储藏物螨，是全球最重要的过敏原来源之一。尽管螨类本身是主要的过敏原来源，但最近的研究表明，与螨类相关的微生物群可能对过敏原产生和人类健康有影响。重要的医学屋尘螨，粉尘螨，已知携带一种属于属的高度丰富的细胞内细菌。螨类和共生体基因的表达分析可以确定促进与这种内共生体相互作用的关键螨类分子途径，并可能揭示这种细菌如何影响螨类过敏原产生和一般生理学。
