Microbiology, School of Biological and Chemical Sciences, University of Galway, Galway, Ireland.
Bat Conservation Ireland, Dublin, Ireland.
Sci Rep. 2024 Aug 23;14(1):19621. doi: 10.1038/s41598-024-70686-y.
Dust samples were collected from Irish homes. House Dust Mite and storage mites were separated from the dust. The microbiome and resistome of mites and originating dust were assessed using a culture-independent approach. The bacterial microbiome of mites and dust were predominantly populated by Staphylococci. There was a highly significant (P = 0.005; Spearman's rank test) correlation between the bacterial microbiome of mites and the dust. One-hundred and eighteen antimicrobial resistance genes (ARGs) were associated with mites and 176 with dust. Both contained ARGs encoding resistance for multi drug resistances, macrolide-lincosamide-streptogramin B, mobile genetic elements, Beta-lactam, Tetracycline and Aminoglycosides. By contrast, 15 ARGs were found for a laboratory-grown strain of Dermatophagoides pteronyssinus. A significant difference (P = 0.03; t test) was found in means between the resistome of mites and the household dust from which they emanated. No significant correlations (P = 0.23 and P = 0.22; Mantel test) were observed between the microbiome and resistome of mite and dust samples. There was not a significant difference (P = 0.54; t-test) between the means of ARGs for homes with and without a history of antibiotic use.
从爱尔兰家庭中收集了灰尘样本。从灰尘中分离出尘螨和储存螨。使用非培养方法评估螨和原始灰尘的微生物组和抗性组。螨和灰尘的细菌微生物组主要由葡萄球菌组成。螨的细菌微生物组与灰尘之间存在高度显著的相关性（P=0.005；Spearman 等级检验）。118 个抗生素耐药基因（ARGs）与螨有关，176 个与灰尘有关。两者都包含编码多药耐药性、大环内酯-林可酰胺-链阳性菌素 B、移动遗传元件、β-内酰胺类、四环素类和氨基糖苷类耐药性的 ARGs。相比之下，在实验室生长的屋尘螨中发现了 15 个 ARGs。螨的抗性组和它们起源的家庭灰尘之间的平均值存在显著差异（P=0.03；t 检验）。未观察到螨和灰尘样本的微生物组和抗性组之间存在显著相关性（P=0.23 和 P=0.22；Mantel 检验）。抗生素使用史的家庭与没有抗生素使用史的家庭之间的 ARGs 平均值没有显著差异（P=0.54；t 检验）。
