State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Mol Oral Microbiol. 2022 Feb;37(1):1-8. doi: 10.1111/omi.12355. Epub 2021 Nov 22.
Dental caries is one of the most prevalent and costly biofilm-dependent oral infectious diseases affecting most of the world's population. Streptococcus mutans, a major extracellular polymeric substance (EPS) producing bacteria in dental plaque, plays a vital role in human dental caries. EPS acts as the framework of dental plaque and promotes bacterial adhesion, cohesion, and environmental stress resistance and hinders the diffusion of nutrients and metabolic products. Since EPS is critical for biofilm lifestyle and virulence of cariogenic bacteria, EPS disruption could be a potential strategy to prevent caries. This review sought to summarize potential strategies to inhibit S. mutans biofilms through EPS disruption. The signal network intervention has a positive effect on S. mutans biofilm disruption, which could be achieved by using cyclic dimeric G/AMP inhibitors, quorum sensing inhibitors, and diffusible signal factors. Besides the enzyme degradation of exopolysaccharides, extracellular DNA, and proteins, other novel strategies, such as nanoparticles and phage therapy, could also promote EPS matrix disruption.
龋齿是一种最普遍且代价高昂的生物膜依赖性口腔传染病，影响着世界上大多数人口。变形链球菌是牙菌斑中一种主要的胞外多聚物（EPS）产生细菌，在人类龋齿中起着至关重要的作用。EPS 作为牙菌斑的框架，促进了细菌的黏附、聚集和环境压力抗性，并阻碍了营养物质和代谢产物的扩散。由于 EPS 对致龋细菌的生物膜生活方式和毒力至关重要，因此 EPS 破坏可能是预防龋齿的一种潜在策略。本综述旨在总结通过 EPS 破坏来抑制变形链球菌生物膜的潜在策略。信号网络干预对变形链球菌生物膜的破坏有积极作用，可通过使用环状二核苷酸 G/AMP 抑制剂、群体感应抑制剂和可扩散信号因子来实现。除了胞外多糖、胞外 DNA 和蛋白质的酶降解外，其他新型策略，如纳米颗粒和噬菌体治疗，也可以促进 EPS 基质的破坏。
