State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Disease, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section of Ren Min Nan Rd., Chengdu, Sichuan, 610041, People's Republic of China.
Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
Int J Implant Dent. 2024 Oct 17;10(1):45. doi: 10.1186/s40729-024-00564-9.
This research endeavors to scrutinize the influence of attachment systems and denture cleaning methodologies on microbial biomass and composition within the realm of implant-supported overdentures, a crucial consideration for patients with dentition defects necessitating such prosthetic solutions.
Employing five polymethyl methacrylate specimens designed to emulate the fitting surfaces of traditional dentures and implant-supported overdentures. Following the polishing of each specimen and the quantification of its roughness, co-cultivation with three distinct microbial strains ensued, culminating in ultrasonic cleaning in water. The bar-clip group, differentiated by the depth of attachment, underwent cleaning employing four diverse methods. Biomass quantities were meticulously recorded both pre and post cleaning interventions, with subsequent data analysis via t-testing and one-way ANOVA, maintaining a significance level of α = 0.05.
The bar-clip groups demonstrated an elevated degree of microbial adhesion, with the deeper locator group exhibiting heightened biomass residue post-cleaning, indicative of increased cleaning complexity. Ultrasonic cleaning predominantly targeted biofilm and deceased bacteria, whereas chemical cleaners primarily reduced the quantity of viable bacteria. The synergistic application of ultrasonics and chemical cleaning treatments yielded the minimal biomass residue.
In contemplating the utilization of dentures milled by dental computer-aided design/manufacturing systems, meticulous pre-use surface polishing is imperative. The extent of biofilm adhesion correlates with the chosen attachment system. This study advocates for the incorporation of ultrasonic cleaning in conjunction with chemical cleaning solutions to optimize the removal of biofilm and live cellular entities in the context of implant-supported overdentures.
本研究旨在探讨附着体系统和义齿清洁方法对种植覆盖义齿中微生物生物量和组成的影响，这是对需要此类修复解决方案的牙列缺损患者的重要考虑因素。
使用五个聚甲基丙烯酸甲酯标本模拟传统义齿和种植覆盖义齿的拟合表面。在对每个标本进行抛光并量化其粗糙度后，与三种不同的微生物菌株进行共培养，最终在水中进行超声清洗。根据附着深度区分的杆夹组采用四种不同的清洁方法进行清洁。在清洁干预前后仔细记录生物量，然后通过 t 检验和单因素方差分析进行数据分析，保持显著水平α=0.05。
杆夹组显示出微生物附着的高度增加，较深的定位器组在清洁后表现出更高的生物量残留，表明清洁的复杂性增加。超声清洗主要针对生物膜和死亡细菌，而化学清洁剂主要减少了活细菌的数量。超声和化学清洁处理的协同应用产生了最小的生物量残留。
在考虑使用由牙科计算机辅助设计/制造系统铣削的义齿时，在使用前仔细进行表面抛光是至关重要的。生物膜附着的程度与所选附着体系统相关。本研究提倡在种植覆盖义齿中结合使用超声清洗和化学清洁溶液，以优化生物膜和活细胞实体的去除。
