从患者标本中直接进行麻疹病毒全基因组测序方法的优化。
Optimisation of methodology for whole genome sequencing of Measles Virus directly from patient specimens.
机构信息
Department of Medical Microbiology and Infectious Diseases, Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.
Department of Medical Microbiology and Infectious Diseases, Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
出版信息
J Virol Methods. 2022 Jan;299:114348. doi: 10.1016/j.jviromet.2021.114348. Epub 2021 Oct 30.
In an era of decreasing genetic diversity of Measles Virus (MeV), effective surveillance requires a higher-resolution genotyping method or whole genome sequencing (WGS) to document elimination. Through optimization of MeV WGS protocol, we developed a MeV-specific probe enrichment method that allows next generation sequencing from clinical specimens. With the probe enrichment method, 70% of specimens can be sequenced at a read depth of greater than 10 reads with minimal off-target sequences.
在麻疹病毒（MeV）遗传多样性降低的时代，有效的监测需要更高分辨率的基因分型方法或全基因组测序（WGS）来记录消除情况。通过优化 MeV WGS 方案，我们开发了一种 MeV 特异性探针富集方法，可从临床标本中进行下一代测序。使用探针富集方法，70%的标本可以以大于 10 个读数的读深进行测序，并且最小化了脱靶序列。
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