State Key Laboratory of Grassland Agro-ecosystems, Lanzhou University, China; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, China; Western China Technology Innovation Center for Grassland Industry, Gansu Province, China; Engineering Research Center of Grassland Industry, Ministry of Education, China; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730000, China.
State Key Laboratory of Grassland Agro-ecosystems, Lanzhou University, China; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, China; Western China Technology Innovation Center for Grassland Industry, Gansu Province, China; Engineering Research Center of Grassland Industry, Ministry of Education, China; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730000, China.
Int J Biol Macromol. 2021 Dec 15;193(Pt A):893-902. doi: 10.1016/j.ijbiomac.2021.10.197. Epub 2021 Oct 30.
In nature, the normal growth, development, and quality of plants are significantly affected by many abiotic stresses, such as drought, salinity, low temperature, and heavy metals. Among heavy metals, copper is an essential element for plant growth and development but also has a toxic effect on plants when its concentration is excessive. Therefore, plants have evolved a complex regulatory network to regulate the balance of copper ions in cells. Heavy metal ATPases (HMAs), which transport heavy metals to intracellular compartments or detoxify heavy metals present at excessive concentrations, have been extensively studied in model plant species. However, no comprehensive and systematic surveys of members of the HMA gene family have been conducted in the model legume species Medicago truncatula. Here, nine putative MtHMAs were identified in the M. truncatula genome. These MtHMAs were phylogenetically divided into two distinct groups. The members in each group had a relatively conserved gene structure and motif composition. The number of introns in the MtHMAs varied from 5 to 16, with the majority of these genes containing 8 introns. The expression patterns showed that MtHMAs exhibit preferential or distinct expression patterns among different tissues. Finally, the expression patterns of the members of this gene family were verified in the leaves and roots of plants under Cu stress. Our findings will be valuable for the functional investigation and application of members of this gene family in M. truncatula and other related legume species.
在自然界中，许多非生物胁迫因素，如干旱、盐度、低温和重金属，都会显著影响植物的正常生长、发育和品质。在重金属中，铜是植物生长和发育所必需的元素，但当其浓度过高时，对植物也有有毒作用。因此，植物已经进化出了一个复杂的调控网络来调节细胞内铜离子的平衡。重金属 ATP 酶（HMAs）将重金属运送到细胞内隔室或解毒过量存在的重金属，在模式植物物种中已经得到了广泛的研究。然而，在模式豆科植物蒺藜苜蓿中，对 HMA 基因家族成员的全面和系统的调查尚未进行。在这里，在蒺藜苜蓿基因组中鉴定出了 9 个假定的 MtHMAs。这些 MtHMAs 在系统发育上分为两个不同的组。每组的成员都具有相对保守的基因结构和基序组成。MtHMAs 的内含子数从 5 到 16 不等，大多数基因含有 8 个内含子。表达模式表明，MtHMAs 在不同组织中表现出优先或独特的表达模式。最后，在 Cu 胁迫下，该基因家族成员在植物的叶片和根中的表达模式得到了验证。我们的研究结果将对该基因家族成员在蒺藜苜蓿和其他相关豆科物种中的功能研究和应用具有重要价值。
