Laboratorio de Biorremediación, Facultad de Ciencias Biológicas, Universidad Autónoma de Coahuila, Torreón 27275, Coahuila, México.
Gus R. Douglass Institute, Department of Biology, West Virginia State University, Institute, WV 25112-1000, USA.
Genes (Basel). 2023 Sep 27;14(10):1877. doi: 10.3390/genes14101877.
The heavy-metal-associated (HMA) proteins are a class of P-type ATPases related to the intracellular transport and detoxification of metals. However, due to a lack of information regarding the HMA gene family in the Cucurbitaceae family, a comprehensive genome-wide analysis of the HMA family was performed in ten Cucurbitaceae species: , , , , , , , , , and . We identified 103 Cucurbit HMA proteins with various members, ranging from 8 () to 14 () across species. The phylogenetic and structural analysis confirmed that the Cucurbitaceae HMA protein family could be further classified into two major clades: Zn/Co/Cd/Pb and Cu/Ag. The GO-annotation-based subcellular localization analysis predicted that all HMA gene family members were localized on membranes. Moreover, the analysis of conserved motifs and gene structure (intron/exon) revealed the functional divergence between clades. The interspecies microsynteny analysis demonstrated that maximum orthologous genes were found between species of the genera. Finally, nine candidate HMA genes were selected, and their expression analysis was carried out via qRT-PCR in root, leaf, flower, and fruit tissues of under arsenic stress. The expression pattern of the CpeHMA genes showed a distinct pattern of expression in root and shoot tissues, with a remarkable expression of and genes from the Cu/Ag clade. Overall, this study provides insights into the functional analysis of the HMA gene family in Cucurbitaceae species and lays down the basic knowledge to explore the role and mechanism of the HMA gene family to cope with arsenic stress conditions.
重金属相关（HMA）蛋白是一类 P 型 ATP 酶，与金属的细胞内运输和解毒有关。然而，由于葫芦科植物中 HMA 基因家族的信息缺乏，我们在 10 种葫芦科物种中进行了 HMA 家族的全基因组分析： 、 、 、 、 、 、 、 、 。我们鉴定了 103 种葫芦科 HMA 蛋白，具有不同的成员，从 8 个（ ）到 14 个（ ），跨物种。系统发生和结构分析证实，葫芦科 HMA 蛋白家族可以进一步分为两个主要分支：Zn/Co/Cd/Pb 和 Cu/Ag。基于 GO 注释的亚细胞定位分析预测，所有 HMA 基因家族成员都定位于膜上。此外，保守基序和基因结构（内含子/外显子）分析揭示了分支之间的功能分化。种间微同线性分析表明， 属的物种之间发现了最大数量的直系同源基因。最后，选择了 9 个候选 HMA 基因，并通过 qRT-PCR 在砷胁迫下的 根、叶、花和果实组织中进行了它们的表达分析。CpeHMA 基因的表达模式在根和茎组织中表现出明显的表达模式，Cu/Ag 分支的 和 基因表达显著。总的来说，这项研究为葫芦科植物 HMA 基因家族的功能分析提供了新的见解，并为探索 HMA 基因家族在应对砷胁迫条件下的作用和机制奠定了基础知识。
