Área Física, Facultad Cs. Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 535, 2000 Rosario, Argentina.
Departamento de Física, Facultad Cs. Exactas, Ingeniería y Agrimensura, Universidad Nacional de Rosario, Av. Pellegrini 250, 2000 Rosario, Argentina.
Comp Biochem Physiol A Mol Integr Physiol. 2022 Feb;264:111109. doi: 10.1016/j.cbpa.2021.111109. Epub 2021 Oct 30.
During diabetes, the characteristic hyperglycemia can induce red blood cell glycation. Several researchers have proposed different protocols to perform an in vitro model to study this phenomenon. In this article, some of the most important in vitro glycation protocols available in the bibliography were compared to each other. The incubation parameters as the suspension medium, glucose concentration, red blood cell concentration, time, and temperature were analyzed. Also, several assays were carried out in our laboratory, and glycated hemoglobin, erythrocyte aggregation and viscoelasticity were determined for the protocol validation. Based on the bibliographic analysis and our experimental results, an optimal protocol for in vitro glycation of red blood cells is presented.
在糖尿病中，特征性的高血糖会引起红细胞糖化。一些研究人员提出了不同的方案来建立体外模型以研究这一现象。在本文中，对文献中一些最重要的体外糖化方案进行了相互比较。对孵育参数（悬浮介质、葡萄糖浓度、红细胞浓度、时间和温度）进行了分析。此外，在我们的实验室还进行了几种测定，以确定糖化血红蛋白、红细胞聚集和粘弹性，从而验证方案。根据文献分析和我们的实验结果，提出了一种优化的红细胞体外糖化方案。
