Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
Division of Medicine, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Japan.
Sci Rep. 2021 Nov 2;11(1):21421. doi: 10.1038/s41598-021-00703-x.
Several clinical studies have been conducted into the practicality and safety of regenerative therapy using hESC/iPSC-retinal pigment epithelium (RPE) as a treatment for the diseases including age-related macular degeneration. These studies used either suspensions of RPE cells or an RPE cell sheet. The cells can be injected using a minimally invasive procedure but the delivery of an intended number of cells at an exact target location is difficult; cell sheets take a longer time to prepare, and the surgical procedure is invasive but can be placed at the target area. In the research reported here, we combined the advantages of the two approaches by producing a quickly formed hiPSC-RPE strip in as short as 2 days. The strip readily expanded into a monolayer sheet on the plate, and after transplantation in nude rats, it showed a potency to partly expand with the correct apical/basal polarity in vivo, although limited in expansion area in the presence of healthy host RPE. The strip could be injected into a target area in animal eyes using a 24G canula tip.
已经有几项临床研究探讨了使用 hESC/iPSC-视网膜色素上皮（RPE）进行再生治疗的实用性和安全性，作为治疗包括年龄相关性黄斑变性在内的疾病的一种方法。这些研究使用了 RPE 细胞悬液或 RPE 细胞片。可以通过微创程序注射细胞，但难以将预定数量的细胞精确地输送到目标位置；细胞片需要更长的时间来准备，并且手术过程具有侵入性，但可以放置在目标区域。在本研究中，我们通过在短短 2 天内生成快速形成的 hiPSC-RPE 条带来结合这两种方法的优点。该条带很容易在平板上扩展成单层片，并且在裸鼠移植后，它显示出在体内部分扩展的能力，具有正确的顶端/基底极性，尽管在存在健康宿主 RPE 的情况下扩展面积有限。该条带可以使用 24G 套管尖端注射到动物眼睛的目标区域。
