Gupta Amit, Gupta Sweety, Rajput Deepak, Durgapal Prashant, Chennatt Jaine John, Kishore Sanjeev, Rao Shalinee, Dhar Puneet, Gupta Manoj, Kant Ravi
Department of Surgery, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India.
Department of Radiation Oncology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India.
J Carcinog. 2021 Sep 4;20:11. doi: 10.4103/jcar.JCar_9_21. eCollection 2021.
Gallbladder cancer is an aggressive cancer with short median survival from the time of diagnosis. Improved understanding of the pathological molecular mechanisms of gallbladder carcinogenesis is important to refine the diagnosis, prognosis, and also to develop novel targeted therapies for patients with advanced Gallbladder cancer (GBC) malignancy. Ki-67 is a marker of cell proliferation and its detection by immunohistochemistry is considered to be an effective method for the detection of prognosis in several tumors. In the present study, we have analyzed expression of immunohistochemical marker Ki-67 in gallbladder carcinoma and its correlation with clinicopathological and radiological parameters.
This prospective observational study was conducted from December 2017 to July 2020. The patients of newly diagnosed gallbladder cancer were enrolled as per the inclusion and exclusion criteria defined in the study protocol. Contrast-enhanced computer tomography of the chest and abdomen and serum tumor markers such as carbohydrate antigen (CA)-19.9, carcinoembryonic antigen, and CA 125 were done. Immunohistochemical expression of Ki-67 was evaluated on biopsy tissue from the gallbladder mass.
Fifty newly diagnosed patients of carcinoma gallbladder were included in the present study. The correlation was studied between clinicodemographic parameters and Ki-67, but no association was found with age, gender, and symptoms. There was a weak positive correlation between Ki-67 and direct bilirubin, serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, and alkaline phosphatase ( = 0.094; 0.126; 0.542; and 0.328, respectively). There was a weak positive correlation between body mass index (Kg/m) and Ki-67, but this correlation was not statistically significant ( = 0.304).
Ki-67 is a marker of proliferation and it correlated with histological differentiation, jaundice and liver function tests, presence of stones, and location of metastases but did not correlate with stage and extent of disease.
胆囊癌是一种侵袭性癌症，自诊断之时起中位生存期较短。更好地理解胆囊癌发生的病理分子机制对于完善诊断、判断预后以及为晚期胆囊癌（GBC）恶性肿瘤患者开发新的靶向治疗方法至关重要。Ki-67是一种细胞增殖标志物，通过免疫组织化学检测它被认为是检测几种肿瘤预后的有效方法。在本研究中，我们分析了免疫组织化学标志物Ki-67在胆囊癌中的表达及其与临床病理和放射学参数的相关性。
本前瞻性观察性研究于2017年12月至2020年7月进行。根据研究方案中定义的纳入和排除标准，纳入新诊断的胆囊癌患者。进行胸部和腹部的对比增强计算机断层扫描以及血清肿瘤标志物检测，如糖类抗原（CA）-19.9、癌胚抗原和CA 125。对胆囊肿物的活检组织进行Ki-67免疫组织化学表达评估。
本研究纳入了50例新诊断的胆囊癌患者。研究了临床人口统计学参数与Ki-67之间的相关性，但未发现与年龄、性别和症状有关联。Ki-67与直接胆红素、血清谷丙转氨酶、血清谷草转氨酶和碱性磷酸酶之间存在弱正相关（分别为r = 0.094；0.126；0.542；和0.328）。体重指数（kg/m²）与Ki-67之间存在弱正相关，但这种相关性无统计学意义（r = 0.304）。
Ki-67是一种增殖标志物，它与组织学分化、黄疸和肝功能检查、结石的存在以及转移部位相关，但与疾病的分期和范围无关。
