de Andrade Reis Régis Josué, Alves Alice Ferreira, Dos Santos Pedro Henrique Dias, Aguiar Kamilla Pereira, da Rocha Letícia Oliveira, da Silveira Silvaldo Felipe, Canellas Luciano Pasqualoto, Olivares Fabio Lopes
Núcleo de Desenvolvimento de Insumos Biológicos para Agricultura (NUDIBA), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil.
Laboratório de Biologia Celular e Tecidual (LBCT), Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil.
World J Microbiol Biotechnol. 2021 Nov 3;37(12):211. doi: 10.1007/s11274-021-03179-z.
A plethora of bacteria-fungal interactions occur on the extended fungal hyphae network in soil. The mycosphere of saprophytic fungi can serve as a bacterial niche boosting their survival, dispersion, and activity. Such ecological concepts can be converted to bioproducts for sustainable agriculture. Accordingly, we tested the hypothesis that the well-characterised beneficial bacterium Serratia marcescens UENF-22GI can enhance plant growth-promoting properties when combined with Trichoderma longibrachiatum UENF-F476. The cultural and cell interactions demonstrated S. marcescens and T. longibrachiatum mutual compatibility. Bacteria cells were able to attach, forming aggregates to biofilms and migrating through the fungal hyphae network. Long-distance bacterial migration through growing hyphae was confirmed using a two-compartment Petri dishes assay. Fungal inoculation increased the bacteria survival rates into the vermicompost substrate over the experimental time. Also, in vitro indolic compound, phosphorus, and zinc solubilisation bacteria activities increased in the presence of the fungus. In line with the ecophysiological bacteria fitness, the bacterium-fungal combination boosted tomato and papaya plantlet growth when applied into the plant substrate under nursery conditions. Mutualistic interaction between mycosphere-colonizing bacterium S. marcescens UENF-22GI and the saprotrophic fungi T. longibrachiatum UENF-F467 increased the ecological fitness of the bacteria alongside with beneficial potential for plant growth. A proper combination and delivery of mutual compatible beneficial bacteria-fungal represent an open avenue for microbial-based products for the biological enrichment of plant substrates in agricultural systems.
土壤中广泛的真菌菌丝网络上存在大量细菌与真菌的相互作用。腐生真菌的菌圈可作为细菌的生态位，促进其生存、扩散和活性。这些生态概念可转化为可持续农业的生物产品。因此，我们验证了以下假设：特性明确的有益细菌粘质沙雷氏菌UENF - 22GI与长枝木霉UENF - F476联合使用时，可增强促进植物生长的特性。培养和细胞相互作用表明粘质沙雷氏菌和长枝木霉相互兼容。细菌细胞能够附着，形成聚集体进而形成生物膜，并通过真菌菌丝网络迁移。使用双室培养皿试验证实了细菌通过生长的菌丝进行长距离迁移。在整个实验期间，接种真菌提高了细菌在蚯蚓堆肥基质中的存活率。此外，在有真菌存在的情况下，体外吲哚化合物、磷和锌的细菌溶解活性增加。与细菌的生态生理适应性一致，在苗圃条件下将细菌 - 真菌组合施用于植物基质时，可促进番茄和木瓜幼苗的生长。定殖于菌圈的粘质沙雷氏菌UENF - 22GI与腐生真菌长枝木霉UENF - F467之间的互利相互作用提高了细菌的生态适应性以及对植物生长的有益潜力。相互兼容的有益细菌 - 真菌的适当组合和施用为农业系统中基于微生物的植物基质生物富集产品开辟了一条途径。
