Division of Animal Genetics, ICAR - Indian Veterinary Research Institute, Izatnangar, Bareilly, UP, 243122, India.
Division of Livestock Production and Management, ICAR - Indian Veterinary Research Institute, Izatnangar, Bareilly, UP, 243122, India.
Anim Genet. 2022 Feb;53(1):68-79. doi: 10.1111/age.13152. Epub 2021 Nov 2.
The live attenuated classical swine fever (CSF) vaccine has been successfully used to prevent and control CSF outbreaks for 6 decades. However, the immune response mechanisms against the vaccine remain poorly understood. Moreover, very few reports exist regarding the breed differences in the response to CSF vaccine. In this study, we generated the peripheral blood mononuclear cell transcriptomes of indigenous Ghurrah and commercial Landrace pig breeds, before and 7 days after CSF vaccination. Subsequently, between and within-breed differential gene expression analyses were carried out. Results revealed large differences in pre-vaccination peripheral blood mononuclear cell transcriptome profiles of the two breeds, which were homogenised 7 days after vaccination. Before vaccination, gene set enrichment analysis showed that pathways related to antigen sensing and innate immune response were enriched in Ghurrah, while pathways related to adaptive immunity were enriched in Landrace. Ghurrah exhibited greater immunomodulation compared to Landrace following the vaccination. In Ghurrah, cell-cycle processes and T-cell response pathways were upregulated after vaccination. However, no pathways were upregulated in Landrace after vaccination. Pathways related to inflammation were downregulated in both the breeds after vaccination. Key regulators of inflammation such as IL1A, IL1B, NFKBIA and TNF genes were strongly downregulated in both the breeds after vaccination. Overall, our results have elucidated the mechanisms of host immune response against CSF vaccination in two distinct breeds and revealed common key genes instrumental in the global immune response to the vaccine.
活疫苗已成功用于预防和控制经典猪瘟 (CSF) 爆发 60 年。然而，人们对疫苗的免疫反应机制仍知之甚少。此外，关于疫苗接种反应的品种差异的报道很少。在这项研究中，我们在 CSF 疫苗接种前后生成了土生土长的 Ghurrah 和商业 Landrace 猪品种的外周血单核细胞转录组。随后，进行了种间和种内差异基因表达分析。结果表明，两种品种的外周血单核细胞转录组在接种前存在很大差异，接种后 7 天则趋于一致。接种前，基因集富集分析表明，Ghurrah 中与抗原感应和固有免疫反应相关的途径富集，而 Landrace 中与适应性免疫相关的途径富集。与 Landrace 相比，Ghurrah 在接种后表现出更强的免疫调节作用。在 Ghurrah 中，接种后细胞周期过程和 T 细胞反应途径上调。然而，Landrace 接种后没有途径上调。接种后两种品种的炎症相关途径均下调。接种后，炎症的关键调节因子如 IL1A、IL1B、NFKBIA 和 TNF 基因在两种品种中均强烈下调。总的来说，我们的研究结果阐明了两种不同品种宿主对 CSF 疫苗接种的免疫反应机制，并揭示了对疫苗产生全球免疫反应的共同关键基因。
