Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P, India.
Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P, India.
Genomics. 2022 Jul;114(4):110427. doi: 10.1016/j.ygeno.2022.110427. Epub 2022 Jul 6.
The present study was undertaken to characterize the distinct immune response in indigenous Ghurrah and exotic Landrace pigs by challenging monocyte-derived macrophages (MDMs) with CSF virus under in-vitro conditions and assessing the variations in the transcriptome profile at 48 h post-infection (hpi). RNA-sequencing was carried out in infected and non-infected MDMs of Ghurrah (n = 3) and Landrace (n = 3) piglets prior- as well as post-stimulation. MDMs of Ghurrah showed greater immune regulation in response to CSF infection with 518 significantly differentially expressed genes (DEG) in infected versus non-infected MDMs, as compared to only 31 DEGs in Landrace MDMs. In Landrace, the principal regulators of inflammation (IL1α, IL1β and TNF) were upregulated in infected cells while in Ghurrah, these were downregulated. Overall, macrophages from indigenous Ghurrah showed more immunological dysregulation in response to virulent CSF virus infection as compared to the exotic Landrace pigs.
本研究旨在通过在体外条件下用 CSF 病毒感染单核细胞衍生的巨噬细胞（MDM），并在感染后 48 小时（hpi）评估转录组谱的变化，来描述土生土长的 Ghurrah 和外来的 Landrace 猪的独特免疫反应。在感染和未感染 Ghurrah（n = 3）和 Landrace（n = 3）仔猪的 MDM 中进行了 RNA 测序，在刺激前后进行了比较。与 Landrace MDM 中仅有 31 个差异表达基因（DEG）相比，Ghurrah 的 MDM 对 CSF 感染的免疫调节作用更大，感染的 MDM 中有 518 个明显差异表达基因（DEG）。在 Landrace 中，感染细胞中炎症的主要调节因子（IL1α、IL1β 和 TNF）上调，而在 Ghurrah 中则下调。总体而言，与外来的 Landrace 猪相比，土生土长的 Ghurrah 中的巨噬细胞对强毒 CSF 病毒感染表现出更多的免疫失调。
