Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, Kurdistan Region, Iraq.
Pol J Vet Sci. 2021 Sep;24(3):365-373. doi: 10.24425/pjvs.2021.138727.
Periodontitis is a highly prevalent, chronic immune-inflammatory disease of the periodontium that results in the periodontium and alveolar bone loss's progressive destruction. In this study, the induction of periodontal disease via retentive ligature, lipopolysaccharide, and their combination at three different times were compared in a rat model. Seventy-two Sprague Dawley rats were distributed into four treatment groups: 1) control group with no treatment; 2) application of 4/0 nylon ligature around second maxillary molars; 3) combination of ligature and LPS injection (ligature-LPS); 4) intragingival injection of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) to the palatal mucosa of the second maxillary molars. Six rats were sacrificed from each group after 7, 14, and 30 days of periodontal disease induction. Alveolar bone loss, attachment loss, number of inflammatory cells, and blood vessels were evaluated histologically. A micro-CT scan was used as a parameter to know the rate of alveolar bone loss. Parametric data were analyzed using two-way ANOVA followed by Bonferroni correction with a significance set at 5%. Non-parametric data were analyzed using Kruskal-Wallis, followed by multiple comparisons with Bonferroni correction. The histological results revealed significant destructive changes in the periodontal tissues and alveolar bone following the ligature and ligature-LPS induction techniques. These changes were evident as early as seven days, maintained until 14 days post-treatment, and declined with time. The ligature technique was effective in inducing acute periodontal disease. The LPS injection technique did not induce alveolar bone loss, and its combination to ligature added insignificant effects.
牙周炎是一种高度流行的、慢性的牙周组织免疫炎症性疾病，导致牙周组织和牙槽骨的进行性破坏。在这项研究中，通过结扎、脂多糖及其组合在三个不同时间点诱导牙周病的大鼠模型进行了比较。将 72 只 Sprague Dawley 大鼠分为四组：1）对照组不治疗；2）在上颌第二磨牙周围应用 4/0 尼龙结扎线；3）结扎和 LPS 注射的组合（结扎 LPS）；4）将牙龈卟啉单胞菌脂多糖（Pg-LPS）注入上颌第二磨牙的腭粘膜。每组有 6 只大鼠在牙周病诱导后 7、14 和 30 天处死。组织学评估牙槽骨丧失、附着丧失、炎症细胞和血管数量。使用微 CT 扫描作为参数来了解牙槽骨丧失的速度。使用双向方差分析，然后使用 Bonferroni 校正进行参数数据分析，显著性水平设为 5%。非参数数据采用 Kruskal-Wallis 分析，然后采用 Bonferroni 校正进行多重比较。组织学结果显示，结扎和结扎 LPS 诱导技术后牙周组织和牙槽骨发生明显的破坏性变化。这些变化早在 7 天就出现了，持续到治疗后 14 天，并随着时间的推移而减少。结扎技术可有效诱导急性牙周病。LPS 注射技术不会引起牙槽骨丧失，与结扎联合使用也没有显著影响。
