Munar-Bestard Marta, Villa Oscar, Ferrà-Cañellas Maria Del Mar, Ramis Joana Maria, Monjo Marta
Group of Cell Therapy and Tissue Engineering, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands (UIB); Balearic Islands Health Research Institute (IdISBa).
Group of Cell Therapy and Tissue Engineering, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands (UIB).
J Vis Exp. 2023 Feb 17(192). doi: 10.3791/64842.
Periodontitis (PD) is a highly prevalent, chronic immune-inflammatory disease of the periodontium, that results in a loss of gingival soft tissue, periodontal ligament, cementum, and alveolar bone. In this study, a simple method of PD induction in rats is described. We provide detailed instructions for placement of the ligature model around the first maxillary molars (M1) and a combination of injections of lipopolysaccharide (LPS), derived from Porphyromonas gingivalis at the mesio-palatal side of the M1. The induction of periodontitis was maintained for 14 days, promoting the accumulation of bacteria biofilm and inflammation. To validate the animal model, IL-1β, a key inflammatory mediator, was determined by an immunoassay in the gingival crevicular fluid (GCF), and alveolar bone loss was calculated using cone beam computed tomography (CBCT). This technique was effective in promoting gingiva recession, alveolar bone loss, and an increase in IL-1β levels in the GCF at the end of the experimental procedure after 14 days. This method was effective in inducing PD, thus being able to be used in studies on disease progression mechanisms and future possible treatments.
牙周炎（PD）是一种牙周组织高度流行的慢性免疫炎症性疾病，会导致牙龈软组织、牙周韧带、牙骨质和牙槽骨丧失。在本研究中，描述了一种在大鼠中诱导牙周炎的简单方法。我们提供了在上颌第一磨牙（M1）周围放置结扎模型以及在M1近中腭侧注射牙龈卟啉单胞菌来源的脂多糖（LPS）的详细说明。牙周炎的诱导持续14天，促使细菌生物膜积累和炎症发生。为验证该动物模型，通过免疫测定法测定龈沟液（GCF）中的关键炎症介质白细胞介素-1β（IL-1β），并使用锥形束计算机断层扫描（CBCT）计算牙槽骨丧失情况。在实验过程14天后的实验末期，该技术有效促进了牙龈退缩、牙槽骨丧失以及GCF中IL-1β水平升高。此方法在诱导牙周炎方面有效，因此可用于疾病进展机制研究和未来可能的治疗研究。
