CircHIPK3 和 CDR1as 环状 RNA 在 2 型糖尿病患者中的循环表达水平。
Circulating expression levels of CircHIPK3 and CDR1as circular-RNAs in type 2 diabetes patients.
机构信息
Department of Biochmistry, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran.
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
出版信息
Mol Biol Rep. 2022 Jan;49(1):131-138. doi: 10.1007/s11033-021-06850-8. Epub 2021 Nov 3.
BACKGROUND
Recent investigations suggested that deregulated levels of Circular RNAs (circRNAs) could be associated with type 2 diabetes mellitus (T2DM) pathogenesis. Accordingly, this study aimed to determine the expression levels of circulating CircHIPK3, CDR1as and their correlation with biochemical parameters in patients with T2DM, pre-diabetes and control subjects.
METHODS AND RESULTS
The expression of circRNAs in peripheral blood was determined using QRT-PCR in 70 patients with T2DM, 60 pre-diabetes and in 69 age and sex matched healthy controls. Moreover, bioinformatics tools were applied to explore and predict the potential interactions between circRNAs and other non-coding RNAs (ncRNAs). Our analysis revealed that the expression level of CircHIPK3 was significantly elevated in T2DM patients compared to healthy participants (P < 0.001) and pre-diabetes subjects (P = 0.018). In addition, ROC analysis suggested that at the cutoff value of 0.24 and the sensitivity and specificity of 50% and 88.4%, respectively, CircHIPK3 could distinguish between T2DM patients and control subjects. Furthermore, it was observed that the expression level of CDR1as is higher in pre-diabetic individuals than healthy individuals (P = 0.004). Finally, Spearman correlation analysis showed that there was a significant correlation between CircHIPK3 and CDR1as expression levels and clinical and anthropometrical parameters such as BMI, systolic and diastolic blood pressure, HbA1c and fasting blood glucose (P < 0.005).
CONCLUSIONS
The data of this study provided evidence that the expression levels of CircHIPK3, CDR1as increased in T2DM and pre-diabetes subjects, respectively.
背景
最近的研究表明，环状 RNA（circRNA）水平失调可能与 2 型糖尿病（T2DM）的发病机制有关。因此，本研究旨在确定循环 CircHIPK3、CDR1as 的表达水平及其与 T2DM、糖尿病前期和对照组患者生化参数的相关性。
方法和结果
在 70 例 T2DM 患者、60 例糖尿病前期患者和 69 名年龄和性别匹配的健康对照者中，采用 QRT-PCR 测定外周血中 circRNAs 的表达。此外，还应用生物信息学工具来探索和预测 circRNAs 与其他非编码 RNA（ncRNA）之间的潜在相互作用。我们的分析表明，与健康参与者（P<0.001）和糖尿病前期患者（P=0.018）相比，T2DM 患者 CircHIPK3 的表达水平显著升高。此外，ROC 分析表明，当截止值为 0.24 时，CircHIPK3 的灵敏度和特异性分别为 50%和 88.4%，可以区分 T2DM 患者和对照组。此外，还观察到糖尿病前期患者的 CDR1as 表达水平高于健康个体（P=0.004）。最后，Spearman 相关分析表明，CircHIPK3 和 CDR1as 的表达水平与 BMI、收缩压和舒张压、HbA1c 和空腹血糖等临床和人体测量参数之间存在显著相关性（P<0.005）。
结论
本研究的数据提供了证据表明，CircHIPK3、CDR1as 的表达水平在 T2DM 和糖尿病前期患者中分别增加。
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