Department of Endocrinology, Cardiovascular and Cerebrovascular Disease Hospital, Ningxia Medical University General Hospital, Yinchuan, Ningxia, China.
Department of Anesthesiology, Shanxi Provincial Cancer Hospital, Xi'an, Shanxi, China.
Ann Clin Lab Sci. 2021 Jan;51(1):90-96.
Type 2 diabetes mellitus (T2DM) is a chronic disease that seriously threatens human health with high incidence and various complications. Circular RNA has become a research hotspot in recent years due to its high stability and wide expression, and has been found to be related to many diseases. The main purpose of this study was to investigate the expression of serum hsa_circ_0054633 in T2DM patients and explore the potential role of serum hsa_circ_0054633 in T2DM diagnosis and its association with clinical characteristics.
This retrospective case-control study enrolled 88 participants including 44 patients with T2DM and 44 age- and sex-matched controls between January 2018 and March 2019 at Ningxia Medical University General Hospital. Serum hsa_circ_0054633 levels were measured using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) with absolute quantification. Baseline information including clinical background, glucose control and biochemical variables were collected. Receiver operating characteristic (ROC) curves were used to evaluate diagnostic effect.
We found that serum expression of hsa_circ_0054633 was dramatically increased in patients with T2DM. Serum hsa_circ_0054633 levels had high sensitivity (75.0%) and specificity (95.5%) for differentiating T2DM. Besides, serum expression of circ_0054633 was significantly correlated with fasting blood glucose (=0.698), hemoglobin A1c (=0.503) and low-density lipoprotein (=0.399).
Serum hsa_circ_0054633 is a potential noninvasive biomarker in discerning T2DM and is associated with changes in blood glucose and lipid.
2 型糖尿病（T2DM）是一种严重威胁人类健康的慢性疾病，具有发病率高、并发症多等特点。近年来，环状 RNA 因其稳定性高、表达广泛而成为研究热点，并且与许多疾病有关。本研究主要目的是探讨 T2DM 患者血清 hsa_circ_0054633 的表达情况，探索血清 hsa_circ_0054633 在 T2DM 诊断中的潜在作用及其与临床特征的关系。
这是一项回顾性病例对照研究，纳入 2018 年 1 月至 2019 年 3 月在宁夏医科大学总医院的 88 名参与者，包括 44 名 T2DM 患者和 44 名年龄和性别匹配的对照者。采用绝对定量实时反转录聚合酶链反应（qRT-PCR）检测血清 hsa_circ_0054633 水平。收集基线信息，包括临床背景、血糖控制和生化变量。采用受试者工作特征（ROC）曲线评估诊断效果。
我们发现 T2DM 患者血清 hsa_circ_0054633 表达显著增加。血清 hsa_circ_0054633 水平对 T2DM 的诊断具有较高的灵敏度（75.0%）和特异性（95.5%）。此外，血清 circ_0054633 表达与空腹血糖（=0.698）、糖化血红蛋白（=0.503）和低密度脂蛋白（=0.399）显著相关。
血清 hsa_circ_0054633 是一种潜在的非侵入性生物标志物，可用于鉴别 T2DM，并且与血糖和血脂变化相关。
