Li Xuejie, Zhao Zhenzhou, Gao Chuanyu, Rao Lixin, Hao Peiyuan, Jian Dongdong, Li Wentao, Tang Haiyu, Li Muwei
Department of Cardiology, People's Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
Department of Cardiology, The First Affiliated Hospital of Zhejiang University, Zhejiang University, Hangzhou, China.
Exp Clin Endocrinol Diabetes. 2017 Jun;125(6):377-383. doi: 10.1055/s-0043-100018. Epub 2017 Apr 13.
This study aims to investigate long noncoding RNA (lncRNA) as biomarker for pre-diabetes and T2DM. LncRNAs in the peripheral blood of 6 healthy individuals and 6 T2DM patients were collected for microarray analysis. Then 5 candidate biomarkers from the differentially expressed lncRNAs were chosen and verified in a larger independent cohort (control group=20; pre-diabetes group=20; and T2DM group=20). The diagnostic capacity of ENST00000550337.1 was further tested in the third cohort (control group, n=60; pre-diabetes group, n=63; and T2DM group, n=64). A total of 17 lncRNAs were found to be differentially expressed between the 2 groups. 14 lncRNAs of these were upregulated in T2DM patients and 3 were downregulated. 5 upregulated lncRNAs were selected as potential biomarkers and verified in the second cohort, and the expression levels of 3 lncRNAs increased gradually from the control group to the pre-diabetes group to the T2DM group. The diagnostic value of ENST00000550337.1 was then tested in the third cohort, and its high diagnostic value for pre-diabetes and T2DM was confirmed. LncRNA ENST00000550337.1 is a potential diagnostic biomarker for pre-diabetes and T2DM.
本研究旨在探讨长链非编码RNA（lncRNA）作为糖尿病前期和2型糖尿病生物标志物的可能性。收集6名健康个体和6名2型糖尿病患者外周血中的lncRNAs进行微阵列分析。然后从差异表达的lncRNAs中选择5种候选生物标志物，并在一个更大的独立队列中进行验证（对照组=20例；糖尿病前期组=20例；2型糖尿病组=20例）。在第三个队列中进一步测试了ENST00000550337.1的诊断能力（对照组，n = 60；糖尿病前期组，n = 63；2型糖尿病组，n = 64）。共发现两组之间有17种lncRNAs差异表达。其中14种lncRNAs在2型糖尿病患者中上调，3种下调。选择5种上调的lncRNAs作为潜在生物标志物并在第二个队列中进行验证，3种lncRNAs的表达水平从对照组到糖尿病前期组再到2型糖尿病组逐渐升高。然后在第三个队列中测试了ENST00000550337.1的诊断价值，证实其对糖尿病前期和2型糖尿病具有较高的诊断价值。LncRNA ENST00000550337.1是糖尿病前期和2型糖尿病的潜在诊断生物标志物。
