Department of Operative Dentistry and Institute of Biomedical Sciences, Dow University of Health Sciences, Karachi, Pakistan.
Department of Restorative Dentistry and Endodontics, College of Dentistry, King Faisal University, Al-Ahsa, Saudi Arabia.
BMC Oral Health. 2021 Nov 3;21(1):562. doi: 10.1186/s12903-021-01904-6.
Matrix metalloproteinases (MMPs) catalyzes the degradation of the extracellular matrix components and have a major role in many physiological processes including wound healing. In the current study, we examined the correlation of baseline MMPs 1, 2, 7, and 9 expressions with periapical wound healing after surgical endodontic treatment.
27 patients aged between 15 and 57 years presenting with chronic apical periodontitis or chronic apical abscess of an anterior tooth with previously attempted or failed root canal treatment were included in this study. During surgical endodontic treatment, tissue from the periapical lesion sample was collected and used for gross histopathological analysis as well as mRNA expression analysis of MMPs 1, 2, 7, and 9. Patients were recalled for follow-up after 6 months to evaluate the healing status both clinically and radiographically and healing was correlated with baseline MMP expression.
Out of 27 patients, healing was observed in 15 patients at the end of 6 months, and in 21 patients after 12 months.. Six patients showed no healing even after 12 months. Analysis of baseline MMP 1, 2, 7, and 9 expression levels with healing status showed the mean relative expression of MMP2 and MMP9 to be considerably increased in the non-healing group as compared to the healing group.
Overexpression of MMP2 and MMP9 may be considered as a potential prognostic biomarker for periapical wound healing after surgical endodontic treatment. However, further studies are desirable to establish its precise relationship with periapical wound healing.
基质金属蛋白酶（MMPs）能催化细胞外基质成分的降解，在许多生理过程中起着重要作用，包括伤口愈合。在本研究中，我们检查了 MMPs1、2、7 和 9 的基线表达与手术根管治疗后根尖周伤口愈合的相关性。
本研究纳入了 27 名年龄在 15 至 57 岁之间的患者，他们患有慢性根尖周炎或前牙慢性根尖脓肿，且之前曾接受过根管治疗或根管治疗失败。在手术根管治疗过程中，从根尖病变组织样本中采集组织，用于大体组织病理学分析以及 MMPs1、2、7 和 9 的 mRNA 表达分析。患者在 6 个月后进行随访以评估临床和放射学上的愈合情况，并将愈合情况与基线 MMP 表达相关联。
在 27 名患者中，有 15 名患者在 6 个月时观察到愈合，有 21 名患者在 12 个月时观察到愈合。6 名患者即使在 12 个月后也没有愈合。对 MMP1、2、7 和 9 的基线表达水平与愈合状态的分析表明，与愈合组相比，非愈合组中 MMP2 和 MMP9 的平均相对表达显著增加。
MMP2 和 MMP9 的过表达可能被认为是手术根管治疗后根尖周伤口愈合的潜在预后生物标志物。然而，需要进一步的研究来确定其与根尖周伤口愈合的确切关系。
