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根尖周肉芽肿的基线基因表达谱与手术根管治疗后根尖周伤口愈合的关系。

Association between the baseline gene expression profile in periapical granuloma and periapical wound healing after surgical endodontic treatment.

机构信息

Department of Restorative Dental Sciences, College of Dentistry, King Faisal University, Al-Ahsa, Saudi Arabia.

Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan.

出版信息

Sci Rep. 2022 Aug 15;12(1):13824. doi: 10.1038/s41598-022-17774-z.

DOI:10.1038/s41598-022-17774-z
PMID:35970906
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9378616/
Abstract

In this study, we have investigated the association between the baseline gene expression profile in periapical granuloma and periapical wound healing after surgical endodontic treatment. Twenty-seven patients aged between 15 and 57 years underwent periapical surgery. The retrieved periapical tissue sample was used for mRNA expression analysis of COL1A1, VTN, ITGA5, IL-4, TNF, ANGPT, VEGFA, and CTGF. All patients were recalled after 6 and 12 months for periapical healing evaluation. Healing was then correlated with baseline gene expression. Healing was observed in 15 patients at the end of 6 months, which increased to 21 patients after 12 months. Six patients showed no healing even after 12 months. Analysis of baseline expression levels of the tested genes with healing status showed the mean relative expression of VTN, VEGFA, ANGPT, TNF, and CTGF to be significantly different (p < 0.05) between the healing group (6 and 12 months) (72.99%) and the non-healing (94.42%) group. Periapical Index scores 3-5 exhibited a positive correlation with ITGA-5 expression. Overexpression of ANGPT and a strong positive correlation between ITGA5 and PAI scores in the non-healing group of patients may suggest these genes to be a potential prognostic biomarker for periapical wound non-healing after surgical endodontic treatment.

摘要

在这项研究中，我们研究了根尖周肉芽肿的基线基因表达谱与手术根管治疗后根尖周伤口愈合之间的关系。27 名年龄在 15 至 57 岁的患者接受了根尖周手术。从根尖周组织样本中提取的 RNA 用于 COL1A1、VTN、ITGA5、IL-4、TNF、ANGPT、VEGFA 和 CTGF 的基因表达分析。所有患者在 6 个月和 12 个月时进行根尖愈合评估。然后将愈合情况与基线基因表达相关联。在 6 个月时，15 名患者出现愈合，12 个月时增加到 21 名。6 名患者即使在 12 个月后也没有愈合。对测试基因的基线表达水平与愈合状态的分析表明，VTN、VEGFA、ANGPT、TNF 和 CTGF 的平均相对表达在愈合组（6 个月和 12 个月）（72.99%）和未愈合组（94.42%）之间存在显著差异（p<0.05）。根尖指数评分 3-5 与 ITGA-5 表达呈正相关。在未愈合组患者中，ANGPT 的过表达和 ITGA5 与 PAI 评分之间的强正相关可能表明这些基因是手术根管治疗后根尖周伤口未愈合的潜在预后生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f53/9378616/86689115e6e3/41598_2022_17774_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f53/9378616/25379a844ecc/41598_2022_17774_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f53/9378616/231ba04f3f67/41598_2022_17774_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f53/9378616/5ad58396c6ac/41598_2022_17774_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f53/9378616/2c5805a334c0/41598_2022_17774_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f53/9378616/86689115e6e3/41598_2022_17774_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f53/9378616/25379a844ecc/41598_2022_17774_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f53/9378616/231ba04f3f67/41598_2022_17774_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f53/9378616/5ad58396c6ac/41598_2022_17774_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f53/9378616/2c5805a334c0/41598_2022_17774_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f53/9378616/86689115e6e3/41598_2022_17774_Fig5_HTML.jpg

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本文引用的文献

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Wound healing: cellular mechanisms and pathological outcomes.伤口愈合：细胞机制和病理结果。
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Endodontic Periapical Lesion: An Overview on the Etiology, Diagnosis and Current Treatment Modalities.牙髓根尖周病损：病因、诊断及当前治疗方法概述。
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