Suzuki Chika, Nomura Masayo, Okutomi Yuki
Sendai Regional Center, Food and Agricultural Materials Inspection Center.
Fertilizer and Feed Inspection Department, Food and Agricultural Materials Inspection Center.
Shokuhin Eiseigaku Zasshi. 2021;62(5):139-147. doi: 10.3358/shokueishi.62.139.
We have developed a quantitative determination method of the concentration of inorganic arsenic in pet foods using a liquid chromatograph-inductively coupled plasma-mass spectrometer (LC-ICP-MS). After adding 2 w/v% TMAH solution to a sample, inorganic arsenic was extracted by heating and the extract was collected by water. The pH of the solution was adjusted, and injected into a LC-ICP-MS to determine the concentration of inorganic arsenic. LC separation was carried out on an ODS column with 10 mmol/L sodium 1-butanesulfonate, 4 mmol/L malonic acid, 4 mmol/L TMAH and 0.05% methanol solution as a mobile phase. A collaborative study was conducted by nine laboratories using dry and wet-type pet foods, formed jerky, dried jerky and biscuit. Dry-type pet food and dried jerky was added with 2 mg/kg of As (III). Wet-type pet food was added with 0.5 mg/kg of As (III). Formed jerky was added with 1 mg/kg of As (III). Biscuit was added with 0.2 mg/kg of As (III). The mean recoveries, repeatabilities and reproducibilities in the form of relative standard deviation (RSD and RSD), and HorRat, were 95.4% to 98.3%, less than 2.9%, less than 9.1%, and 0.22 to 0.51, respectively.
我们开发了一种使用液相色谱-电感耦合等离子体质谱仪（LC-ICP-MS）测定宠物食品中无机砷浓度的定量方法。向样品中加入2 w/v%的四甲基氢氧化铵（TMAH）溶液后，通过加热提取无机砷，并用去离子水收集提取物。调节溶液的pH值，然后注入LC-ICP-MS中以测定无机砷的浓度。液相色谱分离在ODS柱上进行，流动相为含有10 mmol/L 1-丁烷磺酸钠、4 mmol/L丙二酸、4 mmol/L TMAH和0.05%甲醇的溶液。九个实验室使用干型和湿型宠物食品、成型肉干、风干肉干和饼干进行了协同研究。向干型宠物食品和风干肉干中添加了2 mg/kg的As（III）。向湿型宠物食品中添加了0.5 mg/kg的As（III）。向成型肉干中添加了1 mg/kg的As（III）。向饼干中添加了0.2 mg/kg的As（III）。以相对标准偏差（RSD和RSD）以及HorRat表示的平均回收率、重复性和再现性分别为95.4%至98.3%、小于2.9%、小于9.1%以及0.22至0.51。
