建立液相色谱-电感耦合等离子体质谱法测定海产品中砷形态的方法。
Establishment of a method for determination of arsenic species in seafood by LC-ICP-MS.
作者信息
Zmozinski Ariane V, Llorente-Mirandes Toni, López-Sánchez José F, da Silva Márcia M
机构信息
Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Department of Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, E-08028 Barcelona, Spain.
出版信息
Food Chem. 2015 Apr 15;173:1073-82. doi: 10.1016/j.foodchem.2014.10.102. Epub 2014 Oct 28.
An analytical method for determination of arsenic species (inorganic arsenic (iAs), methylarsonic acid (MA), dimethylarsinic acid (DMA), arsenobetaine (AB), trimethylarsine oxide (TMAO) and arsenocholine (AC)) in Brazilian and Spanish seafood samples is reported. This study was focused on extraction and quantification of inorganic arsenic (iAs), the most toxic form. Arsenic speciation was carried out via LC with both anionic and cationic exchange with ICP-MS detection (LC-ICP-MS). The detection limits (LODs), quantification limits (LOQs), precision and accuracy for arsenic species were established. The proposed method was evaluated using eight reference materials (RMs). Arsenobetaine was the main species found in all samples. The total and iAs concentration in 22 seafood samples and RMs ranged between 0.27-35.2 and 0.02-0.71 mg As kg(-1), respectively. Recoveries ranging from 100% to 106% for iAs, based on spikes, were achieved. The proposed method provides reliable iAs data for future risk assessment analysis.
本文报道了一种测定巴西和西班牙海产品样品中砷形态（无机砷（iAs）、甲基砷酸（MA）、二甲基砷酸（DMA）、砷甜菜碱（AB）、三甲基氧化砷（TMAO）和砷胆碱（AC））的分析方法。本研究重点在于提取和定量毒性最强的无机砷（iAs）。通过液相色谱结合阴离子和阳离子交换以及电感耦合等离子体质谱检测（LC-ICP-MS）进行砷形态分析。确定了砷形态的检测限（LOD）、定量限（LOQ）、精密度和准确度。使用八种标准物质（RM）对所提出的方法进行了评估。砷甜菜碱是所有样品中发现的主要形态。22个海产品样品和标准物质中的总砷和iAs浓度分别在0.27 - 35.2和0.02 - 0.71 mg As kg⁻¹之间。基于加标回收率，iAs的回收率在100%至106%之间。所提出的方法为未来的风险评估分析提供了可靠的iAs数据。
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