Mahesh H B, Prasannakumar M K, Manasa K G, Perumal Sampath, Khedikar Yogendra, Kagale Sateesh, Soolanayakanahally Raju Y, Lohithaswa H C, Rao Annabathula Mohan, Hittalmani Shailaja
Department of Genetics and Plant Breeding, College of Agriculture, Mandya, University of Agricultural Sciences, Bengaluru, India.
Department of Plant Pathology, University of Agricultural Sciences, Bengaluru, India.
Front Plant Sci. 2021 Oct 18;12:758119. doi: 10.3389/fpls.2021.758119. eCollection 2021.
Horsegram is a grain legume with excellent nutritional and remedial properties and good climate resilience, able to adapt to harsh environmental conditions. Here, we used a combination of short- and long-read sequencing technologies to generate a genome sequence of 279.12Mb, covering 83.53% of the estimated total size of the horsegram genome, and we annotated 24,521 genes. prediction of DNA repeats showed that approximately 25.04% of the horsegram genome was made up of repetitive sequences, the lowest among the legume genomes sequenced so far. The major transcription factors identified in the horsegram genome were , , , , , , and , suggesting that horsegram is resistant to drought. Interestingly, the genome is abundant in Bowman-Birk protease inhibitors (BBIs), which can be used as a functional food ingredient. The results of maximum likelihood phylogenetic and estimated synonymous substitution analyses suggested that horsegram is closely related to the common bean and diverged approximately 10.17 million years ago. The double-digested restriction associated DNA (ddRAD) sequencing of 40 germplasms allowed us to identify 3,942 high-quality SNPs in the horsegram genome. A genome-wide association study with powdery mildew identified 10 significant associations similar to the and . genes. The reference genome and other genomic information presented in this study will be of great value to horsegram breeding programs. In addition, keeping the increasing demand for food with nutraceutical values in view, these genomic data provide opportunities to explore the possibility of horsegram for use as a source of food and nutraceuticals.
黑豆是一种谷物豆类，具有优异的营养和治疗特性，并且气候适应性强，能够适应恶劣的环境条件。在此，我们结合了短读长和长读长测序技术，生成了一个279.12Mb的基因组序列，覆盖了黑豆基因组估计总大小的83.53%，并注释了24,521个基因。DNA重复序列预测表明，约25.04%的黑豆基因组由重复序列组成，这是迄今为止已测序的豆类基因组中比例最低的。在黑豆基因组中鉴定出的主要转录因子有 、 、 、 、 、 和 ，表明黑豆具有抗旱性。有趣的是，该基因组富含鲍曼-伯克蛋白酶抑制剂（BBIs），可作为功能性食品成分。最大似然系统发育和估计同义替换分析结果表明，黑豆与菜豆密切相关，大约在1017万年前分化。对40份种质进行的双酶切限制性位点关联DNA（ddRAD）测序使我们能够在黑豆基因组中鉴定出3942个高质量单核苷酸多态性（SNP）。一项针对白粉病的全基因组关联研究确定了10个与 和 基因相似的显著关联。本研究中呈现的参考基因组和其他基因组信息将对黑豆育种计划具有重要价值。此外，考虑到对具有营养保健价值食品的需求不断增加，这些基因组数据为探索黑豆作为食品和营养保健品来源的可能性提供了机会。
