Kumar Rahul, Kaundal Sumeet Parkash, Sharma Vikas, Sharma Ashutosh, Singh Gagandeep, Sharma Ram Kumar, Chahota Rakesh Kumar, Sharma Tilak Raj
DAV University, Jalandhar, Punjab, India.
Sant Baba Bhag Singh University, Khiala, Jalandhar, Punjab, India.
Physiol Mol Biol Plants. 2020 Nov;26(11):2255-2266. doi: 10.1007/s12298-020-00898-9. Epub 2020 Oct 27.
Horsegram is an important drought resistant pulse crop from Fabaceae and can be easily grown in dry lands with no irrigation facilities. However, it remained neglected since long and has been considered as orphan legume which requires immediate attention for its improvement and for the development of new promising varieties in future. In the present study, 7352 simple sequence repeat (SSR) markers were developed from the transcriptome data and 150 SSR were randomly synthesized for validation and diversity analysis in a panel of 58 horsegram genotypes. The synthesized primers included all types of repeats spanning direpeats to hexarepeats. Of the validated SSR markers, 33 markers were polymorphic and produced 40 loci which were used to analyze the genetic diversity and structure of horsegram. In total, 130 alleles were produced in a range of 2-9 alleles with maximum alleles produced by primer HTSSR 155. Expected heterozygosity () ranged from 0.03 to 1.00 and observed heterozygosity () ranged from 0.13 to 0.81. Polymorphism information content value ranged from 0.065 to 0.78. Dendrogram based on UPGMA and principal component analysis showed four groups of the 58 genotypes of horsegram. Structure analysis showed three genetic stocks for the analyzed germplasm. Thus, the developed SSRs can be useful in future population genetics analysis, molecular breeding studies and mapping works in horsegram germplasm as well as in related legume species.
黑豆是豆科一种重要的抗旱豆类作物，在没有灌溉设施的旱地也能轻松种植。然而，长期以来它一直被忽视，被视为一种需要立即关注以进行改良并在未来培育新的优良品种的孤生豆科植物。在本研究中，从转录组数据中开发了7352个简单序列重复（SSR）标记，并随机合成了150个SSR用于对58个黑豆基因型进行验证和多样性分析。合成的引物包括从二核苷酸重复到六核苷酸重复的所有重复类型。在经过验证的SSR标记中，33个标记具有多态性，产生了40个位点，用于分析黑豆的遗传多样性和结构。总共产生了130个等位基因，范围为2至9个等位基因，其中引物HTSSR 155产生的等位基因最多。期望杂合度（）范围为0.03至1.00，观察杂合度（）范围为0.13至0.81。多态性信息含量值范围为0.065至0.78。基于非加权组平均法（UPGMA）的聚类图和主成分分析显示，58个黑豆基因型分为四组。结构分析显示所分析的种质有三个遗传库。因此，开发的SSR标记在未来黑豆种质以及相关豆科物种的群体遗传学分析、分子育种研究和图谱绘制工作中可能会有用。
