M Abdelgawad Latifa, Salah Nehal, Sabry Dina, Abdelgwad Marwa
Professor of Medical Laser Applications Department, National Institute of Laser Enhanced Sciences (NILES), Cairo University, Cairo, Egypt.
Medical Laser Applications Department, National Institute of Laser Enhanced Sciences (NILES), Cairo University, Cairo, Egypt.
J Lasers Med Sci. 2021 Jun 24;12:e30. doi: 10.34172/jlms.2021.30. eCollection 2021.
The regeneration of dental pulp tissue using human dental pulp stem cells (HDPSCs) has attracted increasing attention in recent years. Recent studies have suggested that several factors such as photobiomodulation (PBM) and vitamin D affect the proliferation and differentiation of HDPSCs. Therefore, the present study evaluated the effects of PBM and vitamin D on odontogenic differentiation of HDPSCs for dentin -like tissue formation. HDPSCs were collected, isolated, and characterized and then divided into six groups: group I, control; group II, vitamin D (10 Mol); group III, irradiation at 1 J/cm of 810 nm diode laser; group IV, irradiation at 1 J/cm and culture with vitamin D; group V, irradiation at 2 J/cm, and group VI, irradiation at 2 J/cm and culture with vitamin D, cell viability assay was measured through MTT. Alkaline phosphatase (ALP) enzyme activity and mRNA levels of vascular endothelial growth factor (VEGF), bone morphogenic protein-2 (BMP-2), and dentin sialophosphoprotein (DSPP) were also assessed. PBM at 1 and 2 J/cm combined with vitamin D significantly promoted HDPSCs proliferation through MTT assay and odontogenic differentiation through gene expression of VEGF, BMP-2, and DSPP levels ( < 0.0001). PBM at 2 J/cm combined with vitamin D enhanced the HDPSCs proliferation and odontogenic differentiation and thus could be a novel strategy for dentin regeneration in dentistry.
近年来，利用人牙髓干细胞（HDPSCs）进行牙髓组织再生受到了越来越多的关注。最近的研究表明，光生物调节（PBM）和维生素D等多种因素会影响HDPSCs的增殖和分化。因此，本研究评估了PBM和维生素D对HDPSCs向牙本质样组织形成的成牙分化的影响。收集、分离并鉴定HDPSCs，然后将其分为六组：第一组为对照组；第二组为维生素D（10 μmol）组；第三组为用810 nm二极管激光以1 J/cm²照射组；第四组为以1 J/cm²照射并与维生素D共同培养组；第五组为以2 J/cm²照射组；第六组为以2 J/cm²照射并与维生素D共同培养组，通过MTT法检测细胞活力。还评估了碱性磷酸酶（ALP）活性以及血管内皮生长因子（VEGF）、骨形态发生蛋白-2（BMP-2）和牙本质涎磷蛋白（DSPP）的mRNA水平。通过MTT试验，1 J/cm²和2 J/cm²的PBM与维生素D联合使用可显著促进HDPSCs增殖，通过VEGF、BMP-2和DSPP水平的基因表达促进成牙分化（P < 0.0001）。2 J/cm²的PBM与维生素D联合使用可增强HDPSCs增殖和成牙分化，因此可能是牙科牙本质再生的一种新策略。
