Department of Oral Biology, Faculty of Dentistry, The British University in Egypt, El Sherouk City, Egypt.
Department of Pharmacology, Faculty of Dentistry, The British University in Egypt, El Sherouk City, Egypt.
Odontology. 2024 Oct;112(4):1142-1156. doi: 10.1007/s10266-024-00920-6. Epub 2024 Mar 22.
Novel methods and technologies that improve mesenchymal stem cells (MSCs) proliferation and differentiation properties are required to increase their clinical efficacy. Photobiomodulation (PBM) and low-intensity pulsed ultrasound (LIPUS) are two strategies that can be used to enhance the regenerative properties of dental MSCs. This study evaluated the cytocompatibility and osteo/odontogenic differentiation of dental pulp, periodontal ligament, and gingival MSCs after stimulation by either PBM or LIPUS and their combined effect. MTT assay, cell migration assay, osteo/odontogenic differentiation by AR staining and ALP activity, and expression of osteo/odontogenic markers (OPG, OC, RUNX2, DSPP, DMP1) by RT-qPCR were evaluated. Statistical analysis was performed using ANOVA, followed by Tukey's post hoc test, with a p-value of less than 0.05 considered significant. The results showed that combined stimulation by PBM and LIPUS resulted in significantly the highest viability of MSCs, the fastest migration, the most dense AR staining, the most increased ALP activity, and the most elevated levels of osteogenic and odontogenic markers. The synergetic stimulation of PBM and LIPUS can be utilized in cell-based regenerative approaches to promote the properties of dental MSCs.
需要新的方法和技术来提高间充质干细胞（MSCs）的增殖和分化特性，以提高其临床疗效。光生物调节（PBM）和低强度脉冲超声（LIPUS）是两种可用于增强牙齿间充质干细胞再生特性的策略。本研究评估了 PBM 或 LIPUS 刺激后牙髓、牙周韧带和牙龈间充质干细胞的细胞相容性和成骨/成牙分化特性及其联合效应。通过 MTT 检测、细胞迁移检测、碱性磷酸酶（ALP）活性检测、茜素红（AR）染色和 RT-qPCR 检测骨/牙向分化标志物（OPG、OC、RUNX2、DSPP、DMP1）的表达来评估。采用方差分析（ANOVA）进行统计分析，然后进行 Tukey 事后检验，p 值小于 0.05 认为具有统计学意义。结果表明，PBM 和 LIPUS 的联合刺激可显著提高 MSCs 的活力、迁移速度、AR 染色密度、ALP 活性以及成骨和成牙标志物的水平。PBM 和 LIPUS 的协同刺激可用于基于细胞的再生方法，以促进牙齿间充质干细胞的特性。
