Xu Enjun, Li Tao, Chen Qiuli, Wang Zhongxin, Xu Yuanhong
Department of Clinical Laboratory, First Affiliated Hospital of Anhui Medical University, Hefei, China.
Front Med (Lausanne). 2021 Oct 18;8:742067. doi: 10.3389/fmed.2021.742067. eCollection 2021.
The current explored the impact of heat inactivation of blood samples on the results of a particular clinical test and its potential application value during the SARS-CoV-2 pandemic. We have aimed at providing a reference for clinical testing methods during the pandemic. Blood samples were selected from our department's routine clinical examination between January 2021 and June 2021. The levels of these samples for quantitative detection of these indicators in each group ( = 90 cases/group) covered normal reference ranges and medically determined levels. For qualitative testing of the indicators, the specimens were additionally classified as negative, weakly positive, and positive ( = 20 cases/group). The specimens were then inactivated, and the differences in relevant indicators before and after inactivation were evaluated. A statistically significant difference was evident between the levels of TSH, T3, FT4, FT3, AFP, NSE, CYFRA211, IRI, IL-1β, IL-6, IL-8, IL-10, IL-2R, and TNF-α in the non-inactivated group 1 and the inactivated group 1 ( < 0.05). Among them, there was a strong correlation between TSH, T3, FT4, FT3, CYFRA211, IRI, IL-1β, IL-6, IL-8, and IL-2R levels in the two groups ( < 0.05), however, there was no correlation between AFP ( = 0.256) and NSE ( = 0.352) levels between the two groups ( > 0.05). The detected values of low-level AFP (<4 ng/mL), IL-10, and TNF-α after inactivation were all lower than the detection limit. There was not any statistically significant difference in the levels of tumor markers, such as CEA, CA125, CA724, CA199, CA153, and the quantitative levels of T4, Vit. D, HCG, CPS, and five items of hepatitis B virus ( > 0.05). The positive rate of anti-nuclear antibodies after inactivation was not statistically different from the ones observed before inactivation ( > 0.05). Upon correction by the regression equation, the observed levels of TSH, T3, FT4, FT3, CYFRA211, IRI, IL-1β, IL-6, IL-8, and IL-2R were not significantly different from those before inactivation ( > 0.05). The heat inactivation of blood samples had different various effects on different test indicators, and some indicators could be corrected by employing regression equations. This detection method could potentially be employed during the SARS-CoV-2 pandemic, thereby effectively preventing iatrogenic infections.
本研究探讨了血液样本热灭活对一项特定临床试验结果的影响及其在新型冠状病毒肺炎大流行期间的潜在应用价值。我们旨在为大流行期间的临床检测方法提供参考。血液样本选自2021年1月至2021年6月我院科室的常规临床检查。每组（每组 = 90例）用于这些指标定量检测的样本水平涵盖正常参考范围和医学判定水平。对于指标的定性检测，样本另外分为阴性、弱阳性和阳性（每组 = 20例）。然后对样本进行灭活，并评估灭活前后相关指标的差异。未灭活的第1组与灭活的第1组之间促甲状腺激素（TSH）、三碘甲状腺原氨酸（T3）、游离甲状腺素（FT4）、游离三碘甲状腺原氨酸（FT3）、甲胎蛋白（AFP）、神经元特异性烯醇化酶（NSE）、细胞角蛋白片段21-1（CYFRA211）、胰岛素抵抗指数（IRI）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、白细胞介素-10（IL-10）、白细胞介素-2受体（IL-2R）和肿瘤坏死因子-α（TNF-α）水平存在显著统计学差异（P < 0.05）。其中，两组间TSH、T3、FT4、FT3、CYFRA211、IRI、IL-1β、IL-6、IL-8和IL-2R水平存在强相关性（P < 0.05），然而，两组间AFP（P = 0.256）和NSE（P = 0.352）水平无相关性（P > 0.05）。灭活后低水平AFP（<4 ng/mL）、IL-10和TNF-α的检测值均低于检测限。癌胚抗原（CEA）、糖类抗原125（CA125）、糖类抗原724（CA724）、糖类抗原199（CA199）、糖类抗原153（CA153）等肿瘤标志物水平以及甲状腺素（T4）、维生素D（Vit. D）、人绒毛膜促性腺激素（HCG）、癌胚抗原相关细胞黏附分子（CPS）和乙肝五项定量水平无任何显著统计学差异（P > 0.05）。灭活后抗核抗体阳性率与灭活前观察到的阳性率无统计学差异（P > 0.05）。经回归方程校正后，观察到的TSH、T3、FT4、FT3、CYFRA211、IRI、IL-1β、IL-6、IL-8和IL-2R水平与灭活前无显著差异（P > 0.05）。血液样本的热灭活对不同检测指标有不同影响，部分指标可通过回归方程校正。这种检测方法在新型冠状病毒肺炎大流行期间可能具有潜在应用价值，从而有效预防医源性感染。
