Shen Xiaoli, Guo Junhong, Fan Ning, Lai Mingying, Huang Lina, Wang Jiantao, Li Qiang
Department of Glaucoma, Shenzhen Eye Hospital, Shenzhen Eye Hospital Affiliated with Jinan University, School of Optometry, Shenzhen University, Shenzhen, China.
Department of Ophthalmology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, China.
Ann Transl Med. 2021 Sep;9(18):1436. doi: 10.21037/atm-21-4230.
To investigate the protective effect of ultrasound microbubble combined with gross saponins of (GSTT) (a Chinese herb) on glaucomatous optic nerve damage.
Rabbits were randomly divided into five groups. Normal (Group A), high intraocular pressure (IOP, Group B), GSTT (Group C), GSTT + ultrasound (Group D), and GSTT + ultrasound + microbubble destruction (Group E). The high intraocular pressure eye (model eye) was compared to the normal eye (control eye) at 1, 2, and 4 weeks after model establishment. Rabbits were sacrificed 4 weeks later to measure the retina thickness using Cirrus OCT, slit lamp photograph, and fundus photography. The retina and optic nerve of rabbits in each group were collected and the stretched retina were prepared for retinal ganglion cell (RGC) counting, the optic nerve axon was measured, and a transmission electron microscopy was used.
Retina thickness based on Cirrus OCT: mean retinal thickness in Group E was significantly greater than that in Group B, but still thinner than that in Group A. RGCs counts: RGCs counts in Group E were significantly higher than those in Groups B, C, and D but still lower than those in Group A. Quantitative analysis of optic nerve axons: In Group E, the number of optic nerves was increased, diameters of optic nerve axons were decreased, the percentage of optic nerve area occupied by axons was increased, and there were statistically significant differences compared to Groups B, C, and D. Content of GSTT in retina: The content of GSTT in Group E was significantly higher than that in other groups. Observation of the rabbit optic nerves: In Group E, the structure of the myelin sheath of the optic nerve was still intact but less ordered, and the microtubule and microfilament structures in the axons were clear.
Combination of the ultrasound microbubble and GSTT can improve the protective effect of GSTT on optic nerve damage in rabbits with ocular hypertension.
探讨超声微泡联合绞股蓝总皂苷（GSTT，一种中药）对青光眼性视神经损伤的保护作用。
将兔子随机分为五组。正常组（A组）、高眼压组（B组）、绞股蓝总皂苷组（C组）、绞股蓝总皂苷+超声组（D组）和绞股蓝总皂苷+超声+微泡破坏组（E组）。在模型建立后1、2和4周，将高眼压眼（模型眼）与正常眼（对照眼）进行比较。4周后处死兔子，使用Cirrus OCT测量视网膜厚度、进行裂隙灯照相和眼底照相。收集每组兔子的视网膜和视神经，制备拉伸后的视网膜用于视网膜神经节细胞（RGC）计数，测量视神经轴突，并使用透射电子显微镜观察。
基于Cirrus OCT的视网膜厚度：E组的平均视网膜厚度显著大于B组，但仍比A组薄。RGC计数：E组的RGC计数显著高于B、C和D组，但仍低于A组。视神经轴突的定量分析：E组中，视神经数量增加，视神经轴突直径减小，轴突占据的视神经面积百分比增加，与B、C和D组相比有统计学显著差异。视网膜中绞股蓝总皂苷的含量：E组中绞股蓝总皂苷的含量显著高于其他组。兔子视神经观察：E组中，视神经髓鞘结构仍完整但不太规则，轴突中的微管和微丝结构清晰。
超声微泡与绞股蓝总皂苷联合应用可提高绞股蓝总皂苷对高眼压兔视神经损伤的保护作用。
