热休克蛋白A12B（HSPA12B）在非霍奇金淋巴瘤中的表达。
The expression of heat shock protein A12B (HSPA12B) in non-Hodgkin's lymphomas.
作者信息
Huang Yuejiao, Peng Chunlei, Tang Jie, Wang Shitao, Yang Fan, Wang Qiufei, Zhou Li, Yang Lei, Ju Shaoqing
机构信息
Department of Medical Oncology, Affiliated Tumor Hospital of Nantong University, Nantong, China.
Medical School of Nantong University, Nantong, China.
出版信息
Ann Transl Med. 2021 Sep;9(18):1462. doi: 10.21037/atm-21-4185.
BACKGROUND
Heat shock protein A12B (HSPA12B) plays a considerable protective role for cells, tissues, and organs against various noxious conditions. However, the expression of HSPA12B in cancer biology remains controversial. This study aimed to investigate the expression of HSPA12B and its role in cell adhesion mediated drug resistance (CAM-DR) of non-Hodgkin's lymphoma (NHL).
METHODS
In this study, the expression of HSPA12B in NHL was determined by immunohistochemical, and the effect of HSPA12B expression on the prognosis of NHL was analyzed by Kaplan-Meier curves. Then, the transfection technique was used to research the effect of HSPA12B in cell apoptosis. The most important was to study the expression changes of HSPA12B in the adhesion model and the effect of overexpression of HSPA12B on CAM-DR.
RESULTS
We analyzed the relationship between the expression levels of HSPA12B and clinical parameters in NHL. The expression of HSPA12B was directly related to the different NHL variants. We overexpressed HSPA12B in 2 NHL cell lines and found a subsequent reduction in apoptosis. More specifically, we used an adhesion assay to demonstrate that HSPA12B expression was induced in NHL cells when they adhered to fibronectin (FN) or bone marrow stroma cells (BMSCs). Finally, it was revealed that HSPA12B overexpression enhances CAM-DR.
CONCLUSIONS
Our data suggest that HSPA12B may play a functional role in CAM-DR and is thus a potential novel target for NHL treatment.
背景
热休克蛋白A12B（HSPA12B）对细胞、组织和器官抵御各种有害条件发挥着重要的保护作用。然而，HSPA12B在癌症生物学中的表达仍存在争议。本研究旨在探讨HSPA12B在非霍奇金淋巴瘤（NHL）细胞黏附介导的耐药性（CAM-DR）中的表达及其作用。
方法
在本研究中，通过免疫组织化学法测定NHL中HSPA12B的表达，并采用Kaplan-Meier曲线分析HSPA12B表达对NHL预后的影响。然后，利用转染技术研究HSPA12B在细胞凋亡中的作用。最重要的是研究HSPA12B在黏附模型中的表达变化以及HSPA12B过表达对CAM-DR的影响。
结果
我们分析了NHL中HSPA12B表达水平与临床参数之间的关系。HSPA12B的表达与不同的NHL亚型直接相关。我们在2种NHL细胞系中过表达HSPA12B，发现随后细胞凋亡减少。更具体地说，我们通过黏附试验证明，当NHL细胞黏附于纤连蛋白（FN）或骨髓基质细胞（BMSC）时，HSPA12B表达被诱导。最后，结果显示HSPA12B过表达增强了CAM-DR。
结论
我们的数据表明，HSPA12B可能在CAM-DR中发挥功能性作用，因此是NHL治疗的一个潜在新靶点。
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