Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, 48149 Münster, Germany.
J Agric Food Chem. 2021 Nov 17;69(45):13644-13656. doi: 10.1021/acs.jafc.1c04210. Epub 2021 Nov 4.
Dietary biomarkers show great promise for objectively assessing the food intake in humans. In this study, potential urinary biomarkers for red bell pepper intake were identified based on a dietary intervention study and a comprehensive metabolomics approach. Spot urine samples from 14 volunteers were collected in the two phases of the study (control phase: abstaining from any bell pepper/paprika products; case phase: consumption of a defined amount of fresh red bell pepper and abstaining from any further bell pepper/paprika products) and analyzed by high-performance liquid chromatography high-resolution mass spectrometry (HPLC-HRMS). Comparison of the obtained metabolomics data using statistical analysis revealed that the respective urine metabolomes differ significantly, which was attributable to the bell pepper intake. Some of the most discriminating metabolites were selected and isolated from human urine for unequivocal structure elucidation by nuclear magnetic resonance (NMR) spectroscopy. Herein, seven novel glucuronidated metabolites most likely derived from capsanthin and capsianosides were identified, implying their potential application as dietary biomarkers for the entire genus.
膳食生物标志物在客观评估人类食物摄入量方面具有巨大的潜力。在这项研究中，基于饮食干预研究和全面的代谢组学方法，确定了潜在的用于评估摄入红甜椒的尿生物标志物。在研究的两个阶段（对照阶段：不食用任何甜椒/辣椒粉产品；病例阶段：食用一定量的新鲜红甜椒，不食用任何其他甜椒/辣椒粉产品），从 14 名志愿者采集了点尿样，并通过高效液相色谱-高分辨率质谱法（HPLC-HRMS）进行了分析。使用统计分析比较获得的代谢组学数据表明，尿代谢组存在显著差异，这归因于甜椒的摄入。从人尿中选择一些最具区分性的代谢物，并通过核磁共振（NMR）光谱法进行分离，以明确其结构。在此，鉴定出七种新型的可能来源于辣椒素和辣椒苷的葡萄糖醛酸化代谢物，表明它们有可能作为整个属的膳食生物标志物应用。
