Ma Changan, Liu Xiaojie, Zuo Di
School of Health and Social Care, Shanghai Urban Construction Vocational College, Shanghai, 201415, China.
School of Health and Social Care, Shanghai Urban Construction Vocational College, Shanghai, 201415, China.
Biochem Biophys Res Commun. 2021 Oct 27;583:114-120. doi: 10.1016/j.bbrc.2021.10.062.
Energy metabolism is essential for almost all organisms. At the molecular level, adenosine monophosphate activated protein kinase (AMPK) plays a vital role in cellular energy homeostasis. Its molecular characterization in invertebrates, including Daphnia pulex, and the understanding of its role in response to environmental contaminants is limited. In this study, three subunits of AMPK (AMPKα, β, and γ) were cloned in D. pulex, and assigned the GenBank accession numbers MT536758, MT536759, and MT536760, respectively. Their full lengths were 2,000, 1,384, and 2594 bp, respectively, and contained open reading frames of 526, 274, and 580 amino acids, respectively. Bioinformatic analysis revealed that the three AMPK subunits all have features representative of the AMPK superfamily, and were correspondingly clustered with each orthologue branch. The three AMPK subunit genes, AMPKα, β, and γ, had the highest similarity to those of other organisms at 82%, 94%, and 71%, respectively. Nanoplastics significantly increased AMPKα expression, but decreased that of AMPKβ and γ. These results identified AMPKα, β, and γ in D. pulex, and showed that they all encode proteins with conserved functional domains. This study provides basic information on how three types of AMPK in aquatic organisms respond to environmental contaminants.
能量代谢对几乎所有生物体来说都是必不可少的。在分子水平上，腺苷单磷酸激活蛋白激酶（AMPK）在细胞能量稳态中起着至关重要的作用。其在包括大型溞在内的无脊椎动物中的分子特征，以及对其在应对环境污染物中所起作用的了解都很有限。在本研究中，在大型溞中克隆了AMPK的三个亚基（AMPKα、β和γ），并分别被赋予GenBank登录号MT536758、MT536759和MT536760。它们的全长分别为2000、1384和2594 bp，分别包含526、274和580个氨基酸的开放阅读框。生物信息学分析表明，这三个AMPK亚基都具有AMPK超家族的代表性特征，并相应地与每个直系同源分支聚类。三个AMPK亚基基因，AMPKα、β和γ，与其他生物体的基因分别具有82%、94%和71%的最高相似度。纳米塑料显著增加了AMPKα的表达，但降低了AMPKβ和γ的表达。这些结果鉴定了大型溞中的AMPKα、β和γ，并表明它们都编码具有保守功能域的蛋白质。本研究提供了关于水生生物中三种类型的AMPK如何应对环境污染物的基础信息。
