Basic Medical College, College of Chemistry, (Department of Orthopedics) the First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450001, PR China.
Basic Medical College, College of Chemistry, (Department of Orthopedics) the First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450001, PR China.
Biosens Bioelectron. 2022 Jan 15;196:113741. doi: 10.1016/j.bios.2021.113741. Epub 2021 Oct 29.
In this study, a highly ordered three dimensional (3D) DNA nanostructure was self-assembled by label-free DNA nanotweezers, which was used as recognized probe to interact with target. Once the target was recognized by the 3D DNA nanoprobe (3D DNT), DNA nanotweezers opened to release target analog (T1). This recognition process was proceeded in homogeneous solution, which can avoid complex electrode modification and improve reaction efficiency. Then these target analogs were captured by the signal DNA probes (E1) modified on the electrode. In the assistance of Exo III, E1 was digested and the T1 was released to participate in the next cycle to realize signal amplification. Finally, an ultrasensitive carcinoembryonic antigen (CEA) electrochemical biosensing with a detection limit of 4.88 fg mL was developed.
在这项研究中，通过无标记的 DNA 纳米钳自组装了一种高度有序的三维 (3D) DNA 纳米结构，该结构被用作识别探针与靶标相互作用。一旦 3D DNA 纳米探针 (3D DNT) 识别出靶标，DNA 纳米钳就会打开释放靶标类似物 (T1)。这个识别过程在均相溶液中进行，可以避免复杂的电极修饰并提高反应效率。然后，这些靶标类似物被修饰在电极上的信号 DNA 探针 (E1) 捕获。在 Exo III 的协助下，E1 被消化，T1 被释放出来参与下一个循环，以实现信号放大。最终，开发了一种超灵敏的癌胚抗原 (CEA) 电化学生物传感器，其检测限为 4.88 fg mL。
