Berner Berntzen H, Willard-Gallo K E, Munthe E, Jellum E
Scand J Clin Lab Invest Suppl. 1986;184:77-83.
Peripheral blood leukocytes from 30 patients with classic or definite rheumatoid arthritis (RA) and 18 healthy controls were separated into lymphocytes, monocytes and granulocytes and labelled with 35S-methionine. The integral membrane proteins with an amphiphilic nature were separated from hydrophilic proteins by use of the nonionic detergent Triton X-114. Both whole cells and membrane proteins were isotope labelled and separated by high resolution two-dimensional electrophoresis under denaturing conditions. Polypeptide spots were visualised by autoradiography. No consistent differences were found when leukocytes from RA patients were compared to the controls.
将30例典型或确诊类风湿关节炎（RA）患者及18名健康对照者的外周血白细胞分离为淋巴细胞、单核细胞和粒细胞，并用35S-甲硫氨酸进行标记。利用非离子去污剂Triton X-114将具有两亲性的整合膜蛋白与亲水性蛋白分离。全细胞和膜蛋白均进行同位素标记，并在变性条件下通过高分辨率二维电泳进行分离。通过放射自显影观察多肽斑点。将RA患者的白细胞与对照者进行比较时，未发现一致的差异。
