Li Zhenming, Mao Weili, Yao Lei, Zhao Nan, Zhang Yingying, Zhao Meirong, Jin Hangbiao
College of Chemical and Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China.
Department of Pharmacy, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou 324000, PR China.
J Hazard Mater. 2022 Feb 15;424(Pt C):127549. doi: 10.1016/j.jhazmat.2021.127549. Epub 2021 Oct 19.
Studies have demonstrated the worldwide presence of bisphenol A (BPA) and its toxic effects on human health. BPA may exist as several structural isomers, which are byproducts in industrial BPA production. However, nearly nothing is known about the occurrence of BPA isomers in human blood and the partitioning of BPA metabolites between human serum and whole blood. In this study, BPA, BPA-sulfate (BPA-S), and BPA-glucuronide (BPA-G) were quantified in 144 pairs of serum and whole blood samples from Chinese participants. BPA was detected in 115 serum and 121 whole blood samples, with mean concentrations of 0.53 and 0.88 ng/mL, respectively. A structural isomer of BPA, tentatively termed B-BPA, was identified for the first time, and measurable in 53% and 57% of serum (<LOD-1.9 ng/mL) and whole blood (<LOD-1.4 ng/mL) samples, respectively. BPA-S was the predominant BPA metabolite (mean 2.3 and 1.4 ng/mL, respectively), significantly higher (p < 0.01) than BPA-G (1.3 and 0.64 ng/mL) in both serum and whole blood. The calculated partitioning coefficients between serum and whole blood were the highest for B-BPA (mean ± SD, 1.8 ± 0.25), followed by BPA-S (1.6 ± 0.36), BPA-G (1.4 ± 0.37), and BPA (1.3 ± 0.39), indicating their preferential enrichment in the serum fraction. Overall, this study first identifies a BPA isomer, which has not been previously reported in any environmental or human samples. Measuring BPA isomers in human serum and whole blood is critical for accurate human BPA exposure risk assessment.
研究表明，双酚A（BPA）在全球范围内存在，并且对人类健康具有毒性作用。BPA可能以几种结构异构体的形式存在，它们是工业BPA生产中的副产物。然而，关于BPA异构体在人体血液中的存在情况以及BPA代谢物在人血清和全血之间的分配情况，人们几乎一无所知。在本研究中，对来自中国参与者的144对血清和全血样本中的BPA、硫酸双酚A（BPA-S）和双酚A葡萄糖醛酸苷（BPA-G）进行了定量分析。在115份血清样本和121份全血样本中检测到了BPA，其平均浓度分别为0.53和0.88 ng/mL。首次鉴定出一种BPA的结构异构体，暂称为B-BPA，分别在53%的血清样本（<检测限-1.9 ng/mL）和57%的全血样本（<检测限-1.4 ng/mL）中可检测到。BPA-S是主要的BPA代谢物（平均分别为2.3和1.4 ng/mL），在血清和全血中均显著高于BPA-G（1.3和0.64 ng/mL）（p < 0.01）。计算得出的血清和全血之间的分配系数，B-BPA最高（平均值±标准差，1.8 ± 0.25），其次是BPA-S（1.6 ± 0.36）、BPA-G（1.4 ± 0.37）和BPA（1.3 ± 0.39），表明它们在血清部分中优先富集。总体而言，本研究首次鉴定出一种BPA异构体，此前在任何环境或人类样本中均未报道过。测定人血清和全血中的BPA异构体对于准确评估人体BPA暴露风险至关重要。
