School of Marine Sciences, Guangxi University, Nanning, Guangxi, 530000, PR China.
School of Marine Sciences, Guangxi University, Nanning, Guangxi, 530000, PR China; Coral Reef Research Center of China, Nanning, Guangxi, 530000, PR China.
Talanta. 2022 Jan 15;237:122912. doi: 10.1016/j.talanta.2021.122912. Epub 2021 Oct 4.
Quinones are important components participating in various biological processes as well as hazardous substances to human health. Rapid determination of quinones in environmental samples and biofluids is the basis for assessing their health effect. Here, we presented a rapid, straightforward, highly sensitive and environmental-friendly wooden-tip electrospray ionization mass spectrometry (ESI-MS) method for the determination of quinones in PM2.5, urine and serum. An amine group "tag" was introduced to the quinone structure through in situ derivatization with cysteamine to improve ionization efficiency of quinones in wooden-tip ESI-MS. The toothpicks were treated by sharpening and acidification with HNO. Experimental parameters, including sample volume, spray voltage, and spray solvent composition were optimized to be 1 μL, 3.5 kV, and ACN/CHCOOCH (v/v, 9:1), respectively. The limits of detection for the determination of 1,4-benzoquinone, methyl-p-benzoquinone, 1,4-naphthoquinone and 1,4-anthraquinone in ACN under the optimal conditions were 1.00, 0.96, 0.13, 0.16 ng (1.00, 0.96, 0.13, 0.16 μg/mL, sample volume, 1 μL), respectively. This approach was successfully applied to the determination of 1,4-naphthoquinone and 1,4-anthraquinone in complex matrices, including PM2.5, urine and serum without or with minimal sample preparation (LOD range: 0.22-1.48 ng).
醌类是参与各种生物过程的重要组成部分，也是对人类健康有害的物质。快速测定环境样品和生物流体中的醌类物质是评估其健康影响的基础。在这里，我们提出了一种快速、直接、高灵敏度且对环境友好的木制尖端电喷雾电离质谱（ESI-MS）方法，用于测定 PM2.5、尿液和血清中的醌类物质。通过半胱氨酸胺原位衍生化在醌结构中引入一个胺基“标签”，以提高木制尖端 ESI-MS 中醌的电离效率。牙签经过锐化和用 HNO3 酸化处理。实验参数，包括样品体积、喷雾电压和喷雾溶剂组成，分别优化为 1 μL、3.5 kV 和 ACN/CHCOOCH（v/v，9:1）。在最佳条件下，ACN 中 1,4-苯醌、甲基-p-苯醌、1,4-萘醌和 1,4-蒽醌的测定检出限分别为 1.00、0.96、0.13 和 0.16 ng（1.00、0.96、0.13 和 0.16 μg/mL，样品体积 1 μL）。该方法成功应用于复杂基质（包括 PM2.5、尿液和血清）中 1,4-萘醌和 1,4-蒽醌的测定，无需或只需最小的样品制备（LOD 范围：0.22-1.48 ng）。
