Institute of Health and Medical Technology, Hefei Institute of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, 230031, China.
Department of Mechanical Engineering, City University of Hong Kong, Kowloon, Hong Kong, China.
Talanta. 2022 Jan 15;237:122913. doi: 10.1016/j.talanta.2021.122913. Epub 2021 Oct 1.
Sensitivity and credibility detecting histamine (HA) as an important neurotransmitter in biofluids is of importance in analytical science and physiology. Surface-enhanced Raman spectroscopy (SERS) is able to realize the high sensitivity with single molecules level, but providing the high sensitivity for HA with a small cross section remains a challenge. Here we develop the metal complex-based SERS nanoprobe nitrilotriacetic acid-Ni (NTA-Ni) combined with self-assemble Au NPs active substrates for sensitive detection of HA. The NTA-Ni can capture the HA molecules close to Au NPs substrates and then amplify the Raman signals of HA owing to the formation of a complex of NTA-Ni-HA. The self-assemble Au film through the evaporation-driven method can provide the high-density hot spots substrate with high stability and reproducibility. The NTA-Ni decorated Au NPs as nanoprobe responds to HA with 1 μM level of sensitivity. More importantly, the developed SERS nanoprobe composing of NTA-Ni and self-assemble Au NPs can be utilized to detect and monitor the HA spiked into serum, indicating the potential prospect in analysis of HA in complex specimen.
检测生物体液中作为重要神经递质的组氨酸 (HA) 的敏感性和可信度在分析科学和生理学中非常重要。表面增强拉曼光谱 (SERS) 能够实现单分子水平的高灵敏度，但对于具有小横截面积的 HA 提供高灵敏度仍然是一个挑战。在这里，我们开发了基于金属配合物的 SERS 纳米探针乙二胺四乙酸镍 (NTA-Ni)，并结合自组装 Au NPs 活性基底，用于灵敏检测 HA。NTA-Ni 可以捕获靠近 Au NPs 基底的 HA 分子，然后由于 NTA-Ni-HA 复合物的形成，放大 HA 的拉曼信号。通过蒸发驱动方法自组装的 Au 膜可以提供具有高稳定性和重现性的高密度热点基底。用 NTA-Ni 修饰的 Au NPs 作为纳米探针对 HA 的灵敏度达到 1 μM 水平。更重要的是，由 NTA-Ni 和自组装 Au NPs 组成的开发的 SERS 纳米探针可用于检测和监测血清中添加的 HA，表明在复杂样本中分析 HA 的潜在前景。
