Medical School, Institute of Reproductive Medicine, Nantong University, Nantong, 226001, China.
Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009, China.
Talanta. 2022 Jan 15;237:122955. doi: 10.1016/j.talanta.2021.122955. Epub 2021 Oct 9.
Ultrasensitive, multiplex, rapid, and accurate quantitative determination of trace antibiotics remains a challenging issue, which is of importance to public health and safety. Herein, we presented a multiplex strategy based on magnetic nanoparticles and surface-enhanced Raman scattering (SERS) nanotags for simultaneous detection of chloramphenicol (CAP) and tetracycline (TTC). In practice, SERS nanotags based on Raman reporter probes (RRPs) encoded gold-silver core-shell nanostars were used as detection labels for identifying different types of antibiotics, and the magnetic nanoparticles could be separated simply by magnetic force, which significantly improves the detection efficiency, reduces the analysis cost, and simplifies the operation. Our results demonstrate that the as-proposed assay possesses the capacities of high sensitivity and multiplexing with the limits of detection (LODs) for CAP and TTC of 159.49 and 294.12 fg mL, respectively, as well as good stability and reproducibility, and high selectivity and reliability. We believe that this strategy holds a great promising perspective for the detection of trace amounts of antibiotics in microsystems, which is crucial to our life. Additionally, the assay can also be used to detect other illegal additives by altering the appropriate antibodies or aptamers.
超灵敏、多重、快速且准确地定量测定痕量抗生素仍然是一个具有挑战性的问题，这对公共卫生和安全至关重要。在此，我们提出了一种基于磁性纳米粒子和表面增强拉曼散射（SERS）纳米标签的多重策略，用于同时检测氯霉素（CAP）和四环素（TTC）。在实际应用中，基于拉曼报告探针（RRPs）编码的金银核壳纳米星的 SERS 纳米标签被用作识别不同类型抗生素的检测标签，而磁性纳米粒子可以通过磁力简单地分离，这显著提高了检测效率，降低了分析成本，简化了操作。我们的结果表明，该方法具有高灵敏度和多重检测能力，对 CAP 和 TTC 的检测限（LOD）分别为 159.49 和 294.12 fg mL，具有良好的稳定性和重现性，以及高选择性和可靠性。我们相信，这种策略在微系统中痕量抗生素的检测方面具有广阔的前景，这对我们的生活至关重要。此外，通过改变适当的抗体或适体，该测定法还可用于检测其他非法添加剂。
