姜黄通过改变氧化应激、炎症和肠道微生物群来改善二乙基亚硝胺诱导的肝细胞癌。
Curcumae Ameliorates Diethylnitrosamine-Induced Hepatocellular Carcinoma via Alteration of Oxidative Stress, Inflammation and Gut Microbiota.
作者信息
Zhang Yunyan, Li Xuelian, Li Xinghua
机构信息
Department of Laboratory Medicine, The First People's Hospital of Lianyungang City, Lianyungang City, Jiangsu, 222002, People's Republic of China.
Department of Laboratory Medicine, The Fourth People's Hospital of Lianyungang City, Lianyungang City, Jiangsu, 222002, People's Republic of China.
出版信息
J Inflamm Res. 2021 Oct 27;14:5551-5566. doi: 10.2147/JIR.S330499. eCollection 2021.
BACKGROUND
Nonalcoholic fatty liver disease (NAFLD) increased the risk factor of hepatocellular carcinoma (HCC). NAFLD induces the hepatic-related cancer deaths mostly in middle-aged men. NAFLD enhanced the inflammatory reaction and oxidative stress in the hepatic tissue. Curcumae exhibited the anti-inflammatory and antioxidant effects. In this study, we made an attempt to scrutinize the protective effect of curcumae on obesity-induced HCC via alteration of inflammation, oxidative stress and gut microbiota.
METHODS
The rats used in this experiment were Wistar rats, 100 mg/kg intraperitoneal injection of diethylnitrosamine (hepatic carcinogen) was used at 2 weeks. After 6 weeks of the experimental study, the rats were randomly divided into high-fat diet (HFD) with or without curcumae-treated group rats and received the treatment for 22 weeks. Hepatic, non-hepatic, cardiac, antioxidant, pro-inflammatory and inflammatory were estimated at the end of the study. The stools of the experimental rats were collected for estimating the gut microbiota.
RESULTS
Curcumae-treated group rats exposed reduction of the hepatic nodules in hepatic tissue. Curcumae significantly (P<0.001) diminished the level of hepatic parameters and antioxidant parameters in the serum. Curcumae significantly (P<0.001) suppressed the pro-inflammatory cytokines level, viz. interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), interleukin-7 (IL-7) and augmented the level of interleukin-10 (IL-10) in the serum and hepatic tissue. Curcumae significantly (P<0.001) suppressed inflammatory mediators including cyclooxygenase (COX-2), prostaglandin E2 (PGE2) and nuclear factor kappa B (NF-κB) in the serum and hepatic tissue. Furthermore, curcumae increased the gut microbial diversity and richness and decreased the relative abundance of genus and , respectively.
CONCLUSION
Curcumae prevents HFD-induced inflammation during the hepatic carcinoma by modulating the oxidative stress, inflammatory reaction and gut microbiota.
背景
非酒精性脂肪性肝病（NAFLD）增加了肝细胞癌（HCC）的风险因素。NAFLD主要导致中年男性肝脏相关癌症死亡。NAFLD增强了肝组织中的炎症反应和氧化应激。姜黄具有抗炎和抗氧化作用。在本研究中，我们试图通过改变炎症、氧化应激和肠道微生物群来仔细研究姜黄对肥胖诱导的HCC的保护作用。
方法
本实验所用大鼠为Wistar大鼠，在第2周腹腔注射100mg/kg二乙基亚硝胺（肝脏致癌物）。经过6周的实验研究后，将大鼠随机分为高脂饮食（HFD）组和高脂饮食+姜黄治疗组，接受治疗22周。在研究结束时评估肝脏、非肝脏、心脏、抗氧化、促炎和炎症指标。收集实验大鼠的粪便以评估肠道微生物群。
结果
姜黄治疗组大鼠肝脏组织中的肝结节减少。姜黄显著（P<0.001）降低了血清中肝脏参数和抗氧化参数水平。姜黄显著（P<0.001）抑制了促炎细胞因子水平，即白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、白细胞介素-2（IL-2）、白细胞介素-7（IL-7），并提高了血清和肝组织中白细胞介素-10（IL-10）的水平。姜黄显著（P<0.001）抑制了血清和肝组织中的炎症介质，包括环氧化酶（COX-2）、前列腺素E2（PGE2）和核因子κB（NF-κB）。此外，姜黄增加了肠道微生物的多样性和丰富度，并分别降低了属和属的相对丰度。
结论
姜黄通过调节氧化应激、炎症反应和肠道微生物群来预防HFD诱导的肝癌炎症。
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