Rattanapan Yanisa, Duangchan Thitinat, Sai-Ong Thaveesak, Chareonsirisuthigul Takol
Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Hematology and Transfusion Science Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Biology (Basel). 2025 Apr 15;14(4):424. doi: 10.3390/biology14040424.
Type 2 Diabetes Mellitus (T2DM) is a chronic metabolic disorder characterized by insulin resistance and dysregulation of glucose metabolism. MicroRNAs (miRNAs) such as and play critical roles in post-transcriptional regulation of genes involved in these processes, but their specific contributions to T2DM pathogenesis remain unclear. Plasma samples from T2DM patients and non-diabetic controls were analyzed for and expression using microarray and bioinformatics tools. Target genes were predicted, and pathway enrichment analysis was performed to explore biological roles. Differential expression analysis revealed a 2.3-fold upregulation of and a 14.4-fold downregulation of in T2DM patients compared to controls. Predicted targets such as , , and were linked to glucose metabolism and insulin signaling pathways. Enrichment analysis highlighted associations with neuronal signaling, chromatin remodeling, and metabolic regulation pathways. and regulate critical insulin sensitivity and glucose metabolism pathways, making them promising biomarkers and therapeutic targets for managing T2DM. Future studies should validate these findings experimentally to advance miRNA-based interventions for T2DM and its complications.
2型糖尿病（T2DM）是一种慢性代谢紊乱疾病，其特征为胰岛素抵抗和葡萄糖代谢失调。诸如[具体名称未给出]等微小RNA（miRNA）在参与这些过程的基因的转录后调控中发挥关键作用，但其对T2DM发病机制的具体贡献仍不清楚。使用微阵列和生物信息学工具对T2DM患者和非糖尿病对照的血浆样本进行了[具体名称未给出]和[具体名称未给出]表达分析。预测了靶基因，并进行了通路富集分析以探索生物学作用。差异表达分析显示，与对照相比，T2DM患者中[具体名称未给出]上调了2.3倍，[具体名称未给出]下调了14.4倍。预测的靶标如[具体名称未给出]、[具体名称未给出]和[具体名称未给出]与葡萄糖代谢和胰岛素信号通路相关。富集分析突出了与神经元信号传导、染色质重塑和代谢调控通路的关联。[具体名称未给出]和[具体名称未给出]调节关键的胰岛素敏感性和葡萄糖代谢通路，使其成为管理T2DM有前景的生物标志物和治疗靶点。未来的研究应通过实验验证这些发现，以推进基于miRNA的T2DM及其并发症的干预措施。
