Department of Ecology & Environmental System, College of Ecology & Environmental Sciences, Kyungpook National University, Sangju, Gyeongbuk, 37224, Republic of Korea.
Mol Biol Rep. 2022 Jan;49(1):413-420. doi: 10.1007/s11033-021-06887-9. Epub 2021 Nov 5.
A new species within the genus Alternaria was isolated from the leaf spot of Atractylodes ovata in the Mungyeong and Hwabuk-myeon districts of the Gyeongbuk province of Korea. The leaves showed disease symptoms such as circular or irregular leaf spots and brown to dark brown with gray spots at the center. The leaves also showed that concentric rings were surrounded with yellow halos.
Phylogenetic analysis was conducted using the sequence dataset of the internal transcribed spacer region and part of the glyceraldehyde-3-phosphate dehydrogenase. The RNA polymerase II second largest subunit, endopolygalacturonase, Alternaria major allergen gene, anonymous gene region, and translation elongation factor 1-alpha genes were used as well. Results showed that present fungal isolates were distinct from other species of the sect. Alternaria. Morphologically, the present isolates also differed from other members of the sect. Alternaria in their production of solitary conidia or conidial chains (two units) and conidial body features. Similarly, it exhibited moderate pathogenicity in the host plant.
This study described and illustrated A. koreana as a new species and the causal agent of the leaf-spot disease on A. ovata in Korea.
从韩国庆尚北道闻庆市和阴城郡的苍术叶片的叶斑病中分离到链格孢属的一个新种。叶片表现出圆形或不规则的叶斑病症状，中心呈棕色至深棕色，有灰色斑点。叶片还显示出同心环被黄色晕圈包围。
使用内转录间隔区和部分甘油醛-3-磷酸脱氢酶序列数据集进行系统发育分析。还使用 RNA 聚合酶 II 大亚基、内切多聚半乳糖醛酸酶、链格孢主要过敏原基因、匿名基因区和翻译延伸因子 1-α 基因。结果表明，目前的真菌分离株与其他链格孢属的种明显不同。在形态上，这些分离株与链格孢属的其他成员也不同，它们产生单生分生孢子或分生孢子链（两个单位）和分生孢子体特征。同样，它在宿主植物中表现出中等的致病性。
本研究描述并图示了 A. koreana 是一个新种，也是韩国苍术叶片叶斑病的病原菌。
