Institute of Industrial Science, the University of Tokyo, Tokyo, Japan.
Collarborative Research Center, Meisei University, Tokyo, Japan.
Drug Deliv Transl Res. 2022 Feb;12(2):435-443. doi: 10.1007/s13346-021-01050-0. Epub 2021 Nov 5.
Minimally invasive biosensing using microneedles (MNs) is a desirable technology for continuous healthcare monitoring. Among a wide range of MNs, porous MNs are expected to be applied for sampling of interstitial fluids (ISF) by connecting the internal tissue to external measurement devices. In order to realize a continuous measurement of biomarkers in ISF through porous MNs, their integration with a microfluidic chip is a promising approach due to its applicability to micro-total analysis system (μTAS) technology. In this study, we developed a fluidic system to directly interface porous MNs to a microfluidic chip consisting of a capillary pump for the continuous sampling of ISF. The porous and flexible MNs made of PDMS are connected to the microfluidic chip fabricated by standard microelectro-mechanical system (MEMS) processes, showing a continuous flow of phosphate buffered saline (PBS). The developed device will lead to the minimally invasive and continuous biosampling for long-term healthcare monitoring.
使用微针（MNs）进行微创生物传感是连续医疗监测的理想技术。在广泛的 MNs 中，多孔 MNs 有望通过将内部组织与外部测量设备连接起来，用于间质液（ISF）的采样。为了通过多孔 MNs 实现对 ISF 中生物标志物的连续测量，它们与微流控芯片的集成是一种很有前途的方法，因为它适用于微全分析系统（μTAS）技术。在这项研究中，我们开发了一种流体系统，将多孔 MNs 直接与微流控芯片接口，该芯片由用于连续采样 ISF 的毛细管泵组成。由 PDMS 制成的多孔和柔性 MNs 与通过标准微机电系统（MEMS）工艺制造的微流控芯片相连，显示出磷酸盐缓冲盐水（PBS）的连续流动。所开发的器件将用于微创和连续的生物采样，以实现长期的医疗保健监测。
